SeA-SnaP mapping pipeline QC report
run at the BIH CUBI

The reports summarises results of several QC analyses

JavaScript Disabled

MultiQC reports use JavaScript for plots and toolbox functions. It looks like you have JavaScript disabled in your web browser. Please note that many of the report functions will not work as intended.

Report generated on 2020-09-27, 15:07 based on data in: /fast/work/projects/cubi-corona/2020_04_January/GSE157103/mapping

General Statistics

Showing ²⁵²/₂₅₂ rows and ²⁶/₃₇ columns.

Sample Name	M Reads	M Reads Mapped	% GC	Ins. size	≥ 1X	≥ 5X	≥ 10X	≥ 30X	≥ 50X	Median cov	Mean cov	% Aligned	M Aligned	M Total reads	Error rate	5'-3' bias	M Aligned	% Expression Efficiency	# Genes	% rRNA Alignment	% Assigned	M Assigned	% Dups	Error rate	M Non-Primary	M Reads Mapped	% Mapped	% Proper Pairs	% MapQ 0 Reads	M Total seqs	% Aligned	M Aligned	% Dups	% GC	Length	% Failed	M Seqs
SRR12544419	42.7	39.6	49%	269	3.2%	1.0%	0.7%	0.3%	0.2%	0.0X	6.0X	91.6%	34.5	37.7	0.02%	1.19	17.3	79.8%	16559	1.1%	68.5%	14.6	21.8%	0.19%	5.0	34.5	91.6%	91.6%	1.2%	37.7	86.2%	16.2	61.5%	48%	51 bp	20%	18.9
SRR12544420	42.1	39.0	49%	270	3.2%	1.0%	0.7%	0.3%	0.2%	0.0X	5.9X	91.6%	34.0	37.1	0.02%	1.16	17.0	79.7%	16595	1.1%	68.5%	14.4	21.7%	0.21%	5.0	34.0	91.6%	91.6%	1.2%	37.1	86.2%	16.0	60.9%	48%	51 bp	20%	18.6
SRR12544421	25.6	24.4	49%	267	2.5%	0.8%	0.5%	0.2%	0.1%	0.0X	3.3X	94.7%	20.8	21.9	0.02%	1.18	10.4	81.8%	16062	1.3%	67.3%	8.6	19.3%	0.21%	3.6	20.8	94.7%	94.7%	1.4%	21.9	87.5%	9.6	55.4%	48%	51 bp	20%	11.0
SRR12544422	25.1	24.0	49%	268	2.5%	0.8%	0.5%	0.2%	0.1%	0.0X	3.2X	94.7%	20.4	21.6	0.02%	1.20	10.2	81.8%	15928	1.3%	67.3%	8.5	19.1%	0.23%	3.6	20.4	94.7%	94.7%	1.4%	21.6	87.5%	9.4	54.7%	48%	51 bp	20%	10.8
SRR12544423	37.8	36.2	49%	241	2.7%	0.8%	0.5%	0.2%	0.1%	0.0X	4.3X	95.2%	31.6	33.2	0.02%	1.21	15.8	81.9%	15651	0.9%	70.6%	13.3	24.9%	0.20%	4.6	31.6	95.2%	95.2%	1.2%	33.2	89.5%	14.9	64.1%	48%	51 bp	20%	16.6
SRR12544424	37.2	35.6	49%	242	2.6%	0.8%	0.5%	0.2%	0.1%	0.0X	4.2X	95.1%	31.1	32.7	0.03%	1.20	15.5	81.9%	15629	0.9%	70.5%	13.1	24.7%	0.22%	4.5	31.1	95.1%	95.1%	1.2%	32.7	89.5%	14.6	63.7%	48%	51 bp	20%	16.3
SRR12544425	38.6	34.4	49%	269	3.2%	1.0%	0.6%	0.3%	0.1%	0.0X	4.7X	87.6%	29.9	34.1	0.02%	1.13	14.9	74.8%	17293	2.1%	63.8%	12.3	23.8%	0.20%	4.5	29.9	87.6%	87.6%	1.0%	34.1	81.3%	13.9	60.9%	48%	51 bp	20%	17.1
SRR12544426	38.0	33.8	49%	269	3.2%	1.0%	0.6%	0.2%	0.1%	0.0X	4.6X	87.6%	29.4	33.6	0.02%	1.16	14.7	74.8%	17281	2.1%	63.8%	12.1	23.6%	0.22%	4.4	29.4	87.6%	87.6%	1.0%	33.6	81.3%	13.6	60.5%	48%	51 bp	20%	16.8
SRR12544427	40.1	36.1	47%	269	2.8%	0.8%	0.5%	0.2%	0.2%	0.0X	5.0X	89.0%	32.1	36.0	0.02%	1.15	16.0	76.9%	15717	1.5%	67.6%	13.5	25.8%	0.21%	4.0	32.1	89.0%	89.0%	0.8%	36.0	83.6%	15.1	66.8%	46%	51 bp	20%	18.0
SRR12544428	39.5	35.5	47%	269	2.8%	0.8%	0.5%	0.2%	0.1%	0.0X	5.0X	89.0%	31.6	35.5	0.02%	1.15	15.8	76.8%	15645	1.5%	67.5%	13.3	25.6%	0.23%	4.0	31.6	89.0%	89.0%	0.8%	35.5	83.6%	14.8	66.1%	46%	51 bp	20%	17.7
SRR12544429	39.4	35.9	49%	242	2.8%	0.9%	0.6%	0.2%	0.1%	0.0X	4.2X	89.6%	30.3	33.8	0.02%	1.17	15.2	77.7%	16874	1.5%	63.4%	12.5	27.0%	0.20%	5.6	30.3	89.6%	89.6%	1.3%	33.8	82.2%	13.9	63.8%	48%	51 bp	20%	16.9
SRR12544430	38.7	35.3	49%	243	2.8%	0.9%	0.6%	0.2%	0.1%	0.0X	4.1X	89.5%	29.8	33.3	0.03%	1.17	14.9	77.7%	16855	1.5%	63.3%	12.3	26.8%	0.22%	5.5	29.8	89.5%	89.5%	1.3%	33.3	82.1%	13.7	63.7%	48%	51 bp	20%	16.6
SRR12544431	39.9	35.7	49%	256	3.4%	1.1%	0.7%	0.3%	0.2%	0.0X	4.7X	87.6%	30.0	34.3	0.02%	1.18	15.0	74.4%	17736	0.8%	61.2%	12.2	24.0%	0.20%	5.6	30.0	87.6%	87.6%	1.4%	34.3	80.6%	13.8	59.6%	48%	51 bp	20%	17.1
SRR12544432	39.3	35.0	49%	257	3.4%	1.1%	0.7%	0.3%	0.2%	0.0X	4.6X	87.5%	29.5	33.7	0.02%	1.18	14.8	74.3%	17648	0.8%	61.2%	12.0	23.8%	0.22%	5.5	29.5	87.5%	87.5%	1.4%	33.7	80.5%	13.6	58.9%	48%	51 bp	20%	16.9
SRR12544433	38.0	35.0	48%	261	2.8%	0.9%	0.5%	0.2%	0.1%	0.0X	4.6X	91.1%	30.3	33.2	0.02%	1.18	15.1	78.8%	15663	1.8%	66.7%	12.7	25.8%	0.20%	4.8	30.3	91.1%	91.1%	1.3%	33.2	84.9%	14.1	64.3%	47%	51 bp	20%	16.6
SRR12544434	37.4	34.4	48%	262	2.8%	0.9%	0.5%	0.2%	0.1%	0.0X	4.5X	91.1%	29.8	32.7	0.02%	1.17	14.9	78.8%	15687	1.8%	66.7%	12.5	25.7%	0.22%	4.7	29.8	91.1%	91.1%	1.3%	32.7	84.8%	13.9	63.7%	47%	51 bp	20%	16.3
SRR12544435	38.2	36.5	50%	255	3.5%	1.0%	0.6%	0.3%	0.2%	0.0X	5.7X	94.7%	31.2	33.0	0.02%	1.14	15.6	80.0%	16871	0.6%	65.8%	12.6	20.8%	0.21%	5.3	31.2	94.7%	94.7%	1.3%	33.0	87.6%	14.4	58.0%	49%	51 bp	20%	16.5
SRR12544436	37.6	35.9	50%	256	3.5%	1.0%	0.6%	0.3%	0.2%	0.0X	5.6X	94.7%	30.7	32.4	0.02%	1.13	15.3	80.0%	16858	0.6%	65.8%	12.4	20.6%	0.23%	5.2	30.7	94.7%	94.7%	1.3%	32.4	87.6%	14.2	57.7%	49%	51 bp	20%	16.2
SRR12544437	45.6	43.8	50%	265	3.2%	1.0%	0.6%	0.3%	0.2%	0.0X	5.4X	95.4%	36.5	38.3	0.02%	1.17	18.3	82.3%	17017	1.4%	65.8%	15.0	25.5%	0.19%	7.3	36.5	95.4%	95.4%	1.9%	38.3	87.5%	16.8	63.2%	49%	51 bp	20%	19.2
SRR12544438	44.9	43.2	50%	265	3.2%	1.0%	0.6%	0.3%	0.2%	0.0X	5.3X	95.3%	36.0	37.7	0.02%	1.17	18.0	82.3%	17032	1.4%	65.8%	14.8	25.4%	0.21%	7.2	36.0	95.3%	95.3%	1.9%	37.7	87.5%	16.5	62.9%	49%	51 bp	20%	18.9
SRR12544439	39.5	38.3	48%	255	4.0%	1.2%	0.7%	0.3%	0.2%	0.0X	5.2X	96.3%	31.9	33.1	0.02%	1.15	15.9	80.2%	18110	0.8%	64.4%	12.7	20.8%	0.20%	6.4	31.9	96.3%	96.3%	1.7%	33.1	88.3%	14.6	55.1%	47%	51 bp	20%	16.6
SRR12544440	38.8	37.6	48%	256	4.0%	1.1%	0.7%	0.3%	0.2%	0.0X	5.1X	96.3%	31.3	32.5	0.02%	1.15	15.7	80.2%	18173	0.8%	64.4%	12.5	20.6%	0.22%	6.3	31.3	96.3%	96.3%	1.7%	32.5	88.2%	14.4	54.7%	47%	51 bp	20%	16.3
SRR12544441	37.6	36.3	48%	240	3.5%	1.0%	0.6%	0.3%	0.2%	0.0X	5.0X	96.2%	31.9	33.1	0.02%	1.13	15.9	80.9%	16718	1.9%	69.7%	13.1	21.9%	0.20%	4.4	31.9	96.2%	96.2%	1.1%	33.1	90.2%	14.9	59.1%	47%	51 bp	20%	16.6
SRR12544442	37.0	35.7	48%	240	3.5%	1.0%	0.6%	0.3%	0.2%	0.0X	5.0X	96.1%	31.4	32.6	0.02%	1.12	15.7	80.9%	16649	1.9%	69.7%	12.9	21.8%	0.22%	4.3	31.4	96.1%	96.1%	1.1%	32.6	90.1%	14.7	58.6%	47%	51 bp	20%	16.3
SRR12544443	42.4	39.1	47%	254	3.3%	1.0%	0.7%	0.3%	0.2%	0.0X	5.3X	91.1%	33.9	37.3	0.02%	1.17	17.0	78.1%	17538	0.8%	65.7%	13.9	24.0%	0.21%	5.2	33.9	91.1%	91.1%	1.0%	37.3	84.4%	15.7	62.5%	46%	51 bp	20%	18.6
SRR12544444	41.8	38.5	47%	254	3.3%	1.0%	0.6%	0.3%	0.2%	0.0X	5.2X	91.1%	33.4	36.7	0.02%	1.16	16.7	78.0%	17392	0.8%	65.7%	13.7	23.8%	0.23%	5.1	33.4	91.1%	91.1%	1.0%	36.7	84.4%	15.5	61.7%	46%	51 bp	20%	18.3
SRR12544445	40.6	39.0	47%	251	3.6%	1.0%	0.6%	0.3%	0.2%	0.0X	5.5X	95.5%	34.4	36.0	0.02%	1.14	17.2	79.5%	16549	0.5%	69.5%	14.1	21.1%	0.18%	4.6	34.4	95.5%	95.5%	1.1%	36.0	90.1%	16.2	60.2%	46%	51 bp	20%	18.0
SRR12544446	40.0	38.4	47%	252	3.5%	1.0%	0.6%	0.3%	0.2%	0.0X	5.5X	95.4%	33.8	35.4	0.02%	1.16	16.9	79.4%	16521	0.5%	69.5%	13.9	20.9%	0.20%	4.6	33.8	95.4%	95.4%	1.1%	35.4	90.1%	16.0	59.9%	46%	51 bp	20%	17.7
SRR12544447	38.3	34.6	48%	256	3.2%	0.9%	0.6%	0.2%	0.1%	0.0X	4.8X	89.3%	30.5	34.2	0.02%	1.13	15.3	75.6%	16444	1.8%	66.1%	12.7	22.3%	0.21%	4.1	30.5	89.3%	89.3%	1.0%	34.2	83.9%	14.3	61.6%	47%	51 bp	20%	17.1
SRR12544448	37.7	34.1	48%	257	3.2%	0.9%	0.6%	0.2%	0.1%	0.0X	4.7X	89.2%	30.0	33.6	0.02%	1.16	15.0	75.5%	16372	1.8%	66.1%	12.5	22.2%	0.23%	4.0	30.0	89.2%	89.2%	1.0%	33.6	83.9%	14.1	61.2%	47%	51 bp	20%	16.8
SRR12544449	45.4	41.8	48%	269	3.8%	1.1%	0.7%	0.3%	0.2%	0.0X	5.8X	90.9%	35.9	39.5	0.02%	1.13	17.9	77.4%	17889	0.9%	64.6%	14.7	22.3%	0.22%	5.9	35.9	90.9%	90.9%	1.2%	39.5	84.1%	16.6	60.1%	47%	51 bp	20%	19.7
SRR12544450	44.7	41.2	48%	269	3.7%	1.1%	0.7%	0.3%	0.2%	0.0X	5.7X	90.9%	35.3	38.9	0.02%	1.14	17.7	77.4%	17876	0.8%	64.6%	14.4	22.1%	0.24%	5.9	35.3	90.9%	90.9%	1.2%	38.9	84.1%	16.3	59.5%	47%	51 bp	20%	19.4
SRR12544451	31.8	30.4	49%	239	3.0%	0.9%	0.6%	0.2%	0.1%	0.0X	4.2X	94.9%	26.4	27.8	0.02%	1.14	13.2	81.4%	16688	1.6%	68.4%	10.9	20.0%	0.19%	4.0	26.4	94.9%	94.9%	1.1%	27.8	88.2%	12.3	55.9%	48%	51 bp	20%	13.9
SRR12544452	31.3	29.9	49%	239	3.0%	0.9%	0.6%	0.2%	0.1%	0.0X	4.1X	94.9%	25.9	27.3	0.02%	1.13	12.9	81.4%	16622	1.6%	68.3%	10.7	19.8%	0.20%	4.0	25.9	94.9%	94.9%	1.1%	27.3	88.2%	12.0	55.6%	48%	51 bp	20%	13.6
SRR12544453	39.3	38.0	49%	249	3.1%	1.0%	0.6%	0.3%	0.2%	0.0X	5.1X	96.0%	32.8	34.2	0.02%	1.19	16.4	82.7%	16816	0.6%	68.9%	13.6	23.8%	0.19%	5.2	32.8	96.0%	96.0%	1.2%	34.2	89.0%	15.2	60.8%	48%	51 bp	20%	17.1
SRR12544454	38.7	37.3	49%	249	3.1%	1.0%	0.6%	0.3%	0.2%	0.0X	5.0X	96.0%	32.2	33.6	0.02%	1.17	16.1	82.7%	16861	0.6%	68.9%	13.3	23.6%	0.21%	5.1	32.2	96.0%	96.0%	1.1%	33.6	89.0%	14.9	60.4%	48%	51 bp	20%	16.8
SRR12544455	40.8	37.8	47%	246	3.4%	1.1%	0.7%	0.3%	0.2%	0.0X	5.4X	91.7%	33.1	36.1	0.02%	1.13	16.6	78.0%	17117	1.3%	67.1%	13.7	21.6%	0.20%	4.6	33.1	91.7%	91.7%	1.0%	36.1	85.7%	15.5	60.2%	46%	51 bp	20%	18.1
SRR12544456	40.1	37.1	47%	247	3.4%	1.0%	0.7%	0.3%	0.2%	0.0X	5.3X	91.6%	32.6	35.5	0.02%	1.11	16.3	77.9%	17112	1.3%	67.0%	13.4	21.4%	0.21%	4.6	32.6	91.6%	91.6%	1.0%	35.5	85.7%	15.2	59.6%	46%	51 bp	20%	17.8
SRR12544457	39.0	37.5	48%	238	3.0%	0.9%	0.6%	0.3%	0.2%	0.0X	5.4X	95.8%	33.6	35.0	0.02%	1.14	16.8	81.8%	16189	1.4%	72.2%	14.1	21.9%	0.20%	3.9	33.6	95.8%	95.8%	0.9%	35.0	90.5%	15.9	62.4%	47%	51 bp	20%	17.5
SRR12544458	38.3	36.9	48%	239	3.0%	0.9%	0.6%	0.3%	0.2%	0.0X	5.3X	95.7%	33.0	34.5	0.02%	1.15	16.5	81.8%	16132	1.4%	72.2%	13.8	21.7%	0.21%	3.9	33.0	95.7%	95.7%	0.9%	34.5	90.4%	15.6	62.0%	47%	51 bp	20%	17.2
SRR12544459	44.0	42.4	47%	223	3.0%	0.9%	0.6%	0.3%	0.2%	0.0X	5.6X	95.8%	37.4	39.0	0.02%	1.18	18.7	82.6%	15980	1.3%	71.6%	15.7	25.7%	0.19%	5.0	37.4	95.8%	95.8%	1.1%	39.0	90.4%	17.6	65.8%	46%	51 bp	20%	19.5
SRR12544460	43.3	41.7	47%	224	3.0%	0.9%	0.6%	0.3%	0.2%	0.0X	5.5X	95.8%	36.8	38.4	0.02%	1.14	18.4	82.5%	15998	1.3%	71.6%	15.5	25.5%	0.21%	4.9	36.8	95.8%	95.8%	1.1%	38.4	90.3%	17.4	65.5%	46%	51 bp	20%	19.2
SRR12544461	38.1	34.3	46%	249	2.3%	0.7%	0.5%	0.2%	0.1%	0.0X	4.4X	88.5%	29.7	33.6	0.02%	1.21	14.9	78.0%	14931	2.4%	66.1%	12.6	29.9%	0.19%	4.6	29.7	88.5%	88.5%	1.2%	33.6	82.4%	13.8	69.9%	45%	51 bp	20%	16.8
SRR12544462	37.5	33.7	46%	249	2.3%	0.7%	0.4%	0.2%	0.1%	0.0X	4.3X	88.4%	29.2	33.0	0.02%	1.20	14.6	77.9%	14880	2.4%	66.1%	12.4	29.7%	0.21%	4.5	29.2	88.4%	88.4%	1.2%	33.0	82.4%	13.6	69.4%	45%	51 bp	20%	16.5
SRR12544463	36.2	33.1	47%	229	2.8%	0.8%	0.5%	0.2%	0.1%	0.0X	4.1X	90.5%	29.1	32.2	0.02%	1.16	14.6	77.3%	15486	2.6%	67.1%	12.1	25.7%	0.19%	4.0	29.1	90.5%	90.5%	1.0%	32.2	84.9%	13.6	65.0%	46%	51 bp	20%	16.1
SRR12544464	35.6	32.6	47%	229	2.7%	0.8%	0.5%	0.2%	0.1%	0.0X	4.0X	90.5%	28.6	31.7	0.02%	1.16	14.3	77.2%	15433	2.6%	67.0%	11.9	25.6%	0.20%	3.9	28.6	90.5%	90.5%	1.0%	31.7	84.8%	13.4	64.6%	46%	51 bp	20%	15.8
SRR12544465	38.3	36.5	48%	237	3.4%	1.0%	0.6%	0.3%	0.2%	0.0X	5.0X	94.8%	32.6	34.4	0.02%	1.14	16.3	78.8%	16011	1.1%	70.0%	13.4	20.1%	0.19%	3.9	32.6	94.8%	94.8%	0.9%	34.4	89.7%	15.4	60.8%	47%	51 bp	20%	17.2
SRR12544466	37.6	35.8	48%	237	3.4%	0.9%	0.6%	0.3%	0.2%	0.0X	4.9X	94.8%	32.0	33.8	0.02%	1.17	16.0	78.7%	15954	1.1%	70.0%	13.2	19.9%	0.20%	3.8	32.0	94.8%	94.8%	0.9%	33.8	89.7%	15.2	60.3%	47%	51 bp	20%	16.9
SRR12544467	43.0	40.8	49%	272	3.2%	1.0%	0.7%	0.3%	0.2%	0.0X	5.2X	94.2%	34.7	36.9	0.02%	1.21	17.4	81.9%	17299	1.5%	66.7%	14.3	24.4%	0.20%	6.1	34.7	94.2%	94.2%	1.4%	36.9	86.5%	16.0	61.5%	48%	51 bp	20%	18.4
SRR12544468	42.3	40.2	49%	272	3.2%	1.0%	0.7%	0.3%	0.2%	0.0X	5.1X	94.1%	34.2	36.3	0.02%	1.20	17.1	81.8%	17334	1.5%	66.6%	14.1	24.3%	0.22%	6.0	34.2	94.1%	94.1%	1.4%	36.3	86.4%	15.7	60.9%	48%	51 bp	20%	18.2
SRR12544469	34.9	33.5	48%	255	3.1%	0.9%	0.6%	0.2%	0.1%	0.0X	4.2X	95.6%	29.8	31.2	0.02%	1.19	14.9	80.7%	16238	2.2%	70.8%	12.3	21.5%	0.20%	3.7	29.8	95.6%	95.6%	0.9%	31.2	90.0%	14.0	60.9%	47%	51 bp	20%	15.6
SRR12544470	34.4	33.0	48%	255	3.1%	0.9%	0.5%	0.2%	0.1%	0.0X	4.2X	95.5%	29.3	30.7	0.02%	1.18	14.7	80.7%	16171	2.2%	70.8%	12.2	21.4%	0.22%	3.6	29.3	95.5%	95.5%	0.9%	30.7	89.9%	13.8	60.5%	47%	51 bp	20%	15.4
SRR12544471	42.7	41.2	48%	257	3.3%	1.0%	0.6%	0.3%	0.2%	0.0X	5.3X	95.9%	35.1	36.6	0.02%	1.20	17.6	81.5%	16835	1.3%	67.7%	14.5	24.8%	0.20%	6.1	35.1	95.9%	95.9%	1.5%	36.6	89.1%	16.3	62.6%	47%	51 bp	20%	18.3
SRR12544472	42.1	40.6	48%	257	3.3%	1.0%	0.6%	0.3%	0.2%	0.0X	5.2X	95.9%	34.6	36.1	0.02%	1.21	17.3	81.5%	16770	1.3%	67.7%	14.2	24.6%	0.22%	6.0	34.6	95.9%	95.9%	1.5%	36.1	89.1%	16.1	62.2%	47%	51 bp	20%	18.0
SRR12544473	39.1	37.6	48%	242	3.1%	0.9%	0.6%	0.3%	0.2%	0.0X	5.1X	95.7%	33.0	34.4	0.02%	1.19	16.5	80.6%	15964	1.1%	69.8%	13.6	22.8%	0.20%	4.6	33.0	95.7%	95.7%	1.2%	34.4	90.2%	15.5	62.2%	47%	51 bp	20%	17.2
SRR12544474	38.5	37.0	48%	242	3.1%	0.9%	0.6%	0.2%	0.2%	0.0X	5.0X	95.7%	32.5	33.9	0.02%	1.20	16.2	80.6%	15912	1.1%	69.8%	13.4	22.6%	0.21%	4.6	32.5	95.7%	95.7%	1.2%	33.9	90.2%	15.3	62.1%	47%	51 bp	20%	17.0
SRR12544475	31.4	30.2	48%	229	2.1%	0.7%	0.4%	0.2%	0.1%	0.0X	3.3X	95.5%	25.7	26.9	0.03%	1.17	12.8	83.4%	15006	1.3%	69.0%	10.8	27.1%	0.20%	4.5	25.7	95.5%	95.5%	1.7%	26.9	89.0%	12.0	65.8%	47%	51 bp	20%	13.4
SRR12544476	30.9	29.7	48%	230	2.1%	0.6%	0.4%	0.2%	0.1%	0.0X	3.2X	95.5%	25.2	26.4	0.03%	1.15	12.6	83.3%	14976	1.3%	69.0%	10.6	26.8%	0.22%	4.4	25.2	95.5%	95.5%	1.6%	26.4	89.0%	11.8	65.2%	47%	51 bp	20%	13.2
SRR12544477	41.1	39.3	48%	251	3.4%	1.0%	0.6%	0.3%	0.2%	0.0X	4.9X	95.0%	34.0	35.8	0.02%	1.12	17.0	80.5%	17032	1.2%	67.7%	13.9	28.4%	0.22%	5.3	34.0	95.0%	95.0%	1.1%	35.8	88.2%	15.8	63.8%	47%	51 bp	30%	17.9
SRR12544478	40.5	38.7	48%	251	3.4%	1.0%	0.6%	0.2%	0.2%	0.0X	4.8X	95.0%	33.5	35.3	0.03%	1.08	16.8	80.5%	16980	1.2%	67.7%	13.7	28.2%	0.23%	5.2	33.5	95.0%	95.0%	1.1%	35.3	88.1%	15.5	63.3%	47%	51 bp	30%	17.6
SRR12544479	30.9	29.4	48%	272	2.5%	0.8%	0.5%	0.2%	0.1%	0.0X	3.9X	94.6%	25.7	27.2	0.02%	1.17	12.8	82.7%	15827	1.2%	70.3%	10.9	23.6%	0.20%	3.7	25.7	94.6%	94.6%	1.2%	27.2	88.5%	12.0	62.2%	47%	51 bp	20%	13.6
SRR12544480	30.4	29.0	48%	273	2.5%	0.7%	0.5%	0.2%	0.1%	0.0X	3.8X	94.5%	25.3	26.7	0.02%	1.17	12.6	82.7%	15793	1.2%	70.3%	10.7	23.4%	0.22%	3.7	25.3	94.5%	94.5%	1.2%	26.7	88.5%	11.8	61.6%	47%	51 bp	20%	13.4
SRR12544481	38.2	36.6	48%	244	3.7%	1.0%	0.6%	0.2%	0.1%	0.0X	4.3X	95.3%	31.6	33.1	0.02%	1.11	15.8	76.2%	16392	1.7%	64.7%	12.4	23.1%	0.20%	5.1	31.6	95.3%	95.3%	1.2%	33.1	88.4%	14.6	60.6%	47%	51 bp	20%	16.6
SRR12544482	37.6	36.0	48%	244	3.6%	1.0%	0.6%	0.2%	0.1%	0.0X	4.2X	95.2%	31.0	32.6	0.03%	1.11	15.5	76.2%	16375	1.7%	64.7%	12.1	22.9%	0.22%	5.0	31.0	95.2%	95.2%	1.2%	32.6	88.3%	14.4	60.2%	47%	51 bp	20%	16.3
SRR12544483	36.1	34.3	50%	257	2.9%	0.9%	0.6%	0.2%	0.1%	0.0X	4.7X	94.2%	29.7	31.5	0.02%	1.22	14.9	81.4%	16987	0.9%	68.1%	12.3	22.7%	0.22%	4.6	29.7	94.2%	94.2%	1.1%	31.5	87.4%	13.8	60.2%	49%	51 bp	20%	15.8
SRR12544484	35.5	33.7	50%	258	2.9%	0.9%	0.6%	0.2%	0.1%	0.0X	4.6X	94.2%	29.2	31.0	0.02%	1.19	14.6	81.4%	16955	0.9%	68.1%	12.1	22.5%	0.23%	4.5	29.2	94.2%	94.2%	1.1%	31.0	87.4%	13.5	59.7%	49%	51 bp	20%	15.5
SRR12544485	36.3	34.8	49%	257	3.3%	1.0%	0.6%	0.2%	0.1%	0.0X	4.5X	95.4%	30.6	32.1	0.02%	1.12	15.3	80.8%	16657	1.1%	69.1%	12.6	26.8%	0.21%	4.2	30.6	95.4%	95.4%	0.8%	32.1	88.7%	14.2	61.1%	48%	51 bp	30%	16.1
SRR12544486	35.8	34.3	49%	257	3.3%	0.9%	0.6%	0.2%	0.1%	0.0X	4.4X	95.3%	30.1	31.6	0.02%	1.14	15.1	80.8%	16636	1.1%	69.1%	12.3	26.6%	0.22%	4.2	30.1	95.3%	95.3%	0.8%	31.6	88.6%	14.0	60.9%	48%	51 bp	30%	15.8
SRR12544487	62.9	60.7	49%	245	3.9%	1.2%	0.8%	0.4%	0.2%	0.0X	8.6X	95.9%	53.4	55.6	0.02%	1.16	26.7	82.2%	18053	1.0%	70.0%	22.0	28.7%	0.21%	7.3	53.4	95.9%	95.9%	0.9%	55.6	89.5%	24.9	67.2%	48%	51 bp	20%	27.8
SRR12544488	62.0	59.8	49%	246	3.9%	1.2%	0.8%	0.4%	0.2%	0.0X	8.5X	95.9%	52.6	54.8	0.02%	1.15	26.3	82.2%	17968	1.0%	69.9%	21.7	28.6%	0.22%	7.2	52.6	95.9%	95.9%	0.9%	54.8	89.5%	24.5	66.8%	48%	51 bp	20%	27.4
SRR12544489	29.3	26.7	48%	250	2.4%	0.7%	0.4%	0.2%	0.1%	0.0X	3.3X	89.8%	23.4	26.1	0.02%	1.27	11.7	78.1%	15806	1.6%	67.0%	9.8	24.5%	0.20%	3.3	23.4	89.8%	89.8%	0.9%	26.1	83.8%	10.9	63.4%	47%	51 bp	20%	13.0
SRR12544490	28.8	26.2	48%	250	2.4%	0.7%	0.4%	0.2%	0.1%	0.0X	3.2X	89.7%	23.0	25.6	0.03%	1.25	11.5	78.0%	15810	1.6%	67.0%	9.7	24.3%	0.22%	3.2	23.0	89.7%	89.7%	0.9%	25.6	83.8%	10.7	62.8%	47%	51 bp	20%	12.8
SRR12544491	17.5	16.8	48%	235	2.2%	0.6%	0.4%	0.1%	0.1%	0.0X	2.2X	95.4%	14.8	15.5	0.02%	1.16	7.4	81.1%	14788	1.2%	70.2%	6.1	17.0%	0.19%	2.0	14.8	95.4%	95.4%	1.0%	15.5	89.6%	7.0	52.9%	47%	51 bp	20%	7.8
SRR12544492	17.1	16.4	48%	236	2.2%	0.6%	0.3%	0.1%	0.1%	0.0X	2.2X	95.4%	14.5	15.2	0.02%	1.15	7.2	81.1%	14679	1.2%	70.2%	6.0	16.8%	0.20%	1.9	14.5	95.4%	95.4%	1.0%	15.2	89.5%	6.8	52.4%	47%	51 bp	20%	7.6
SRR12544493	28.6	25.2	48%	244	2.1%	0.6%	0.4%	0.2%	0.1%	0.0X	2.9X	86.7%	22.1	25.5	0.03%	1.26	11.1	75.0%	14820	1.0%	65.3%	9.3	26.2%	0.22%	3.0	22.1	86.7%	86.7%	0.9%	25.5	81.3%	10.4	65.4%	47%	51 bp	20%	12.8
SRR12544494	28.1	24.7	48%	245	2.1%	0.6%	0.4%	0.2%	0.1%	0.0X	2.8X	86.6%	21.8	25.1	0.03%	1.19	10.9	75.0%	14759	1.0%	65.3%	9.2	26.0%	0.24%	3.0	21.8	86.6%	86.6%	0.9%	25.1	81.3%	10.2	64.8%	47%	51 bp	20%	12.6
SRR12544495	33.8	32.2	49%	246	2.0%	0.6%	0.4%	0.2%	0.1%	0.0X	3.3X	94.5%	27.4	29.0	0.03%	1.15	13.7	84.7%	15342	2.5%	68.8%	11.6	33.5%	0.24%	4.8	27.4	94.5%	94.5%	1.4%	29.0	87.0%	12.6	69.2%	48%	51 bp	20%	14.5
SRR12544496	33.2	31.7	49%	247	2.0%	0.6%	0.4%	0.2%	0.1%	0.0X	3.3X	94.4%	26.9	28.5	0.03%	1.15	13.5	84.6%	15430	2.5%	68.7%	11.4	33.3%	0.25%	4.7	26.9	94.4%	94.4%	1.4%	28.5	87.0%	12.4	68.7%	48%	51 bp	20%	14.3
SRR12544497	34.6	33.0	48%	269	3.0%	0.9%	0.6%	0.2%	0.2%	0.0X	5.0X	94.7%	29.3	31.0	0.02%	1.14	14.7	80.9%	16258	1.0%	70.8%	12.3	20.4%	0.20%	3.6	29.3	94.7%	94.7%	0.9%	31.0	89.2%	13.8	60.2%	47%	51 bp	20%	15.5
SRR12544498	34.1	32.4	48%	270	3.0%	0.9%	0.6%	0.2%	0.1%	0.0X	4.9X	94.6%	28.9	30.5	0.02%	1.16	14.4	80.9%	16171	1.0%	70.8%	12.1	20.2%	0.21%	3.6	28.9	94.6%	94.6%	0.9%	30.5	89.2%	13.6	59.6%	47%	51 bp	20%	15.3
SRR12544499	40.2	39.0	50%	238	2.4%	0.7%	0.5%	0.2%	0.1%	0.0X	4.6X	96.5%	33.9	35.1	0.03%	1.12	16.9	85.2%	15636	1.6%	71.7%	14.4	30.4%	0.21%	5.1	33.9	96.5%	96.5%	1.2%	35.1	90.2%	15.8	67.9%	49%	51 bp	20%	17.6
SRR12544500	39.6	38.4	50%	239	2.4%	0.7%	0.5%	0.2%	0.1%	0.0X	4.5X	96.4%	33.3	34.5	0.03%	1.14	16.7	85.1%	15595	1.6%	71.6%	14.2	30.2%	0.23%	5.0	33.3	96.4%	96.4%	1.2%	34.5	90.1%	15.6	67.6%	49%	51 bp	20%	17.3
SRR12544501	36.5	35.3	48%	234	2.7%	0.8%	0.5%	0.2%	0.1%	0.0X	4.4X	96.1%	30.5	31.8	0.02%	1.16	15.3	83.4%	16458	1.8%	69.6%	12.7	24.7%	0.20%	4.7	30.5	96.1%	96.1%	1.2%	31.8	89.2%	14.2	63.3%	47%	51 bp	20%	15.9
SRR12544502	35.9	34.7	48%	235	2.7%	0.8%	0.5%	0.2%	0.1%	0.0X	4.4X	96.1%	30.0	31.2	0.02%	1.15	15.0	83.4%	16349	1.8%	69.6%	12.5	24.6%	0.22%	4.7	30.0	96.1%	96.1%	1.2%	31.2	89.2%	13.9	62.7%	47%	51 bp	20%	15.6
SRR12544503	34.1	30.7	49%	229	1.8%	0.6%	0.4%	0.2%	0.1%	0.0X	3.1X	88.7%	26.5	29.9	0.03%	1.22	13.2	78.9%	14598	3.9%	66.4%	11.3	38.2%	0.21%	4.2	26.5	88.7%	88.7%	1.3%	29.9	82.7%	12.3	72.5%	48%	51 bp	20%	14.9
SRR12544504	33.5	30.2	49%	230	1.8%	0.6%	0.4%	0.2%	0.1%	0.0X	3.1X	88.7%	26.0	29.3	0.03%	1.24	13.0	78.8%	14445	3.9%	66.4%	11.1	37.9%	0.22%	4.2	26.0	88.7%	88.7%	1.3%	29.3	82.7%	12.1	71.9%	48%	51 bp	20%	14.7
SRR12544505	35.2	32.4	47%	228	2.8%	0.8%	0.5%	0.2%	0.1%	0.0X	4.5X	90.9%	28.6	31.5	0.02%	1.21	14.3	79.2%	16055	2.6%	67.9%	12.0	25.3%	0.19%	3.8	28.6	90.9%	90.9%	0.8%	31.5	84.9%	13.4	63.4%	46%	51 bp	20%	15.7
SRR12544506	34.6	31.8	47%	228	2.7%	0.8%	0.5%	0.2%	0.1%	0.0X	4.4X	90.9%	28.1	30.9	0.02%	1.18	14.0	79.1%	15992	2.6%	68.0%	11.8	25.1%	0.21%	3.7	28.1	90.9%	90.9%	0.8%	30.9	84.9%	13.1	63.1%	46%	51 bp	20%	15.5
SRR12544507	39.9	38.5	47%	236	2.7%	0.8%	0.5%	0.2%	0.1%	0.0X	4.8X	95.9%	34.2	35.7	0.02%	1.21	17.1	83.3%	15697	3.4%	72.3%	14.4	29.3%	0.19%	4.2	34.2	95.9%	95.9%	0.9%	35.7	90.2%	16.1	68.4%	46%	51 bp	20%	17.8
SRR12544508	39.3	37.8	47%	237	2.7%	0.8%	0.5%	0.2%	0.1%	0.0X	4.7X	95.9%	33.6	35.1	0.02%	1.25	16.8	83.3%	15669	3.4%	72.3%	14.2	29.1%	0.21%	4.2	33.6	95.9%	95.9%	0.9%	35.1	90.1%	15.8	67.9%	46%	51 bp	20%	17.5
SRR12544509	35.5	31.6	48%	246	2.3%	0.7%	0.4%	0.2%	0.1%	0.0X	3.4X	87.3%	26.8	30.7	0.03%	1.18	13.4	76.6%	15943	2.4%	62.9%	11.2	31.5%	0.21%	4.8	26.8	87.3%	87.3%	1.2%	30.7	80.2%	12.3	69.3%	47%	51 bp	20%	15.4
SRR12544510	34.9	31.1	48%	246	2.2%	0.7%	0.4%	0.2%	0.1%	0.0X	3.3X	87.2%	26.4	30.2	0.03%	1.18	13.2	76.5%	15887	2.4%	62.8%	11.0	31.3%	0.22%	4.7	26.4	87.2%	87.2%	1.2%	30.2	80.1%	12.1	68.6%	47%	51 bp	20%	15.1
SRR12544511	38.9	35.0	47%	245	3.0%	0.9%	0.5%	0.2%	0.1%	0.0X	4.2X	88.7%	30.8	34.8	0.02%	1.20	15.4	75.3%	16305	2.8%	65.5%	12.8	26.5%	0.19%	4.2	30.8	88.7%	88.7%	1.0%	34.8	83.2%	14.5	65.1%	46%	51 bp	20%	17.4
SRR12544512	38.3	34.4	47%	246	3.0%	0.9%	0.5%	0.2%	0.1%	0.0X	4.1X	88.6%	30.3	34.2	0.03%	1.20	15.2	75.3%	16309	2.8%	65.5%	12.5	26.3%	0.21%	4.1	30.3	88.6%	88.6%	1.0%	34.2	83.1%	14.2	64.6%	46%	51 bp	20%	17.1
SRR12544513	37.6	34.1	50%	255	1.9%	0.6%	0.4%	0.2%	0.1%	0.0X	2.9X	88.5%	27.5	31.1	0.03%	1.14	13.8	78.2%	15271	3.1%	60.1%	11.3	39.7%	0.21%	6.5	27.5	88.5%	88.5%	2.0%	31.1	79.8%	12.4	73.8%	49%	51 bp	30%	15.6
SRR12544514	37.0	33.4	50%	255	1.9%	0.6%	0.4%	0.2%	0.1%	0.0X	2.9X	88.4%	27.1	30.6	0.03%	1.16	13.5	78.2%	15209	3.1%	60.1%	11.1	39.4%	0.22%	6.4	27.1	88.4%	88.4%	2.0%	30.6	79.7%	12.2	73.5%	49%	51 bp	30%	15.3
SRR12544515	34.1	30.4	52%	267	1.6%	0.5%	0.3%	0.2%	0.1%	0.0X	2.7X	87.0%	25.2	29.0	0.04%	1.18	12.6	77.0%	15442	1.6%	61.0%	10.4	40.1%	0.25%	5.1	25.2	87.0%	87.0%	1.6%	29.0	79.1%	11.5	73.9%	50%	51 bp	20%	14.5
SRR12544516	33.5	29.8	52%	268	1.6%	0.5%	0.3%	0.2%	0.1%	0.0X	2.7X	87.0%	24.8	28.5	0.04%	1.20	12.4	77.0%	15442	1.6%	61.0%	10.2	39.8%	0.26%	5.0	24.8	87.0%	87.0%	1.6%	28.5	79.1%	11.3	73.4%	50%	51 bp	20%	14.2
SRR12544517	20.5	19.0	51%	353	1.0%	0.3%	0.2%	0.1%	0.0%	0.0X	1.5X	90.2%	14.6	16.2	0.04%	1.24	7.3	83.0%	13027	1.7%	58.8%	6.0	49.3%	0.24%	4.4	14.6	90.2%	90.2%	2.8%	16.2	79.2%	6.4	76.2%	50%	51 bp	20%	8.1
SRR12544518	20.2	18.6	51%	353	1.0%	0.3%	0.2%	0.1%	0.0%	0.0X	1.5X	90.2%	14.3	15.9	0.04%	1.20	7.2	83.0%	12991	1.7%	58.8%	5.9	49.0%	0.26%	4.3	14.3	90.2%	90.2%	2.8%	15.9	79.2%	6.3	76.0%	50%	51 bp	20%	8.0
SRR12544519	50.6	45.6	49%	256	3.0%	1.0%	0.6%	0.3%	0.2%	0.0X	5.2X	88.3%	37.4	42.4	0.03%	1.16	18.7	76.1%	17187	1.3%	60.9%	15.4	29.2%	0.21%	8.2	37.4	88.3%	88.3%	2.0%	42.4	81.0%	17.2	67.0%	48%	51 bp	20%	21.2
SRR12544520	49.8	44.9	49%	257	3.0%	1.0%	0.6%	0.3%	0.2%	0.0X	5.1X	88.3%	36.9	41.7	0.03%	1.15	18.4	76.0%	17147	1.3%	60.9%	15.2	29.0%	0.23%	8.1	36.9	88.3%	88.3%	2.0%	41.7	81.0%	16.9	66.6%	48%	51 bp	20%	20.9
SRR12544521	18.6	16.9	48%	261	1.7%	0.5%	0.3%	0.1%	0.1%	0.0X	2.0X	89.7%	15.1	16.9	0.03%	1.25	7.6	78.7%	13794	0.9%	69.4%	6.5	23.0%	0.21%	1.8	15.1	89.7%	89.7%	0.8%	16.9	84.7%	7.1	61.0%	47%	51 bp	20%	8.4
SRR12544522	18.4	16.7	48%	262	1.7%	0.5%	0.3%	0.1%	0.1%	0.0X	2.0X	89.7%	14.9	16.6	0.03%	1.26	7.4	78.7%	13774	0.9%	69.3%	6.4	22.8%	0.23%	1.8	14.9	89.7%	89.7%	0.8%	16.6	84.7%	7.0	60.2%	47%	51 bp	20%	8.3
SRR12544523	32.6	29.5	46%	258	3.2%	0.9%	0.6%	0.2%	0.1%	0.0X	3.9X	89.4%	26.1	29.2	0.02%	1.16	13.0	75.0%	16520	0.6%	65.7%	10.7	20.8%	0.19%	3.4	26.1	89.4%	89.4%	0.8%	29.2	83.8%	12.2	58.9%	45%	51 bp	20%	14.6
SRR12544524	32.0	29.0	46%	259	3.2%	0.9%	0.6%	0.2%	0.1%	0.0X	3.8X	89.3%	25.7	28.7	0.02%	1.15	12.8	75.0%	16558	0.7%	65.6%	10.5	20.6%	0.21%	3.3	25.7	89.3%	89.3%	0.8%	28.7	83.8%	12.0	58.3%	45%	51 bp	20%	14.4
SRR12544525	35.6	33.9	47%	247	2.6%	0.8%	0.5%	0.2%	0.1%	0.0X	4.3X	94.8%	30.2	31.9	0.02%	1.12	15.1	82.5%	15652	1.0%	72.1%	12.8	25.2%	0.21%	3.7	30.2	94.8%	94.8%	0.9%	31.9	89.3%	14.2	65.2%	46%	51 bp	20%	15.9
SRR12544526	35.0	33.4	47%	248	2.6%	0.8%	0.5%	0.2%	0.1%	0.0X	4.3X	94.7%	29.7	31.4	0.03%	1.16	14.9	82.5%	15646	1.0%	72.1%	12.6	25.0%	0.22%	3.6	29.7	94.7%	94.7%	0.9%	31.4	89.2%	14.0	64.5%	46%	51 bp	20%	15.7
SRR12544527	41.4	41.0	50%	260	2.0%	0.6%	0.4%	0.2%	0.1%	0.0X	3.8X	98.7%	33.9	34.4	0.03%	1.13	17.0	87.5%	14960	1.6%	69.5%	14.4	34.5%	0.20%	7.0	33.9	98.7%	98.7%	2.0%	34.4	91.0%	15.7	73.1%	49%	51 bp	20%	17.2
SRR12544528	40.7	40.3	50%	260	2.0%	0.6%	0.4%	0.2%	0.1%	0.0X	3.8X	98.6%	33.4	33.9	0.03%	1.13	16.7	87.4%	14940	1.6%	69.5%	14.2	34.3%	0.21%	6.9	33.4	98.6%	98.6%	2.0%	33.9	91.0%	15.4	72.8%	49%	51 bp	20%	16.9
SRR12544529	46.0	45.1	49%	272	3.4%	1.0%	0.6%	0.3%	0.2%	0.0X	5.3X	97.7%	39.2	40.1	0.02%	1.17	19.6	83.5%	16604	2.0%	71.0%	16.3	27.8%	0.19%	6.0	39.2	97.7%	97.7%	1.2%	40.1	91.4%	18.3	66.8%	47%	51 bp	20%	20.0
SRR12544530	45.4	44.5	49%	272	3.4%	1.0%	0.6%	0.3%	0.2%	0.0X	5.2X	97.7%	38.6	39.5	0.02%	1.16	19.3	83.5%	16556	2.0%	70.9%	16.1	27.7%	0.20%	5.9	38.6	97.7%	97.7%	1.2%	39.5	91.4%	18.1	66.5%	47%	51 bp	20%	19.8
SRR12544531	41.0	40.0	50%	231	2.1%	0.6%	0.4%	0.2%	0.1%	0.0X	3.2X	97.0%	31.8	32.8	0.03%	1.07	15.9	84.6%	15620	1.6%	64.4%	13.2	42.9%	0.19%	8.1	31.8	97.0%	97.0%	2.7%	32.8	88.7%	14.6	74.9%	49%	51 bp	20%	16.4
SRR12544532	40.3	39.3	50%	231	2.1%	0.6%	0.4%	0.2%	0.1%	0.0X	3.2X	96.9%	31.3	32.3	0.03%	1.11	15.7	84.5%	15664	1.6%	64.4%	13.0	42.6%	0.20%	8.0	31.3	96.9%	96.9%	2.7%	32.3	88.7%	14.3	74.5%	49%	51 bp	20%	16.2
SRR12544533	53.5	52.6	51%	288	2.4%	0.8%	0.6%	0.3%	0.2%	0.0X	5.1X	98.0%	42.1	42.9	0.03%	1.24	21.0	87.4%	17157	2.6%	64.9%	17.4	38.4%	0.21%	10.6	42.1	98.0%	98.0%	2.2%	42.9	88.0%	18.9	73.7%	50%	51 bp	20%	21.5
SRR12544534	52.7	51.9	51%	289	2.3%	0.8%	0.5%	0.3%	0.2%	0.0X	5.1X	98.0%	41.5	42.3	0.03%	1.25	20.7	87.4%	17102	2.6%	65.0%	17.1	38.2%	0.22%	10.4	41.5	98.0%	98.0%	2.2%	42.3	88.0%	18.6	73.0%	50%	51 bp	20%	21.2
SRR12544535	42.8	41.8	49%	271	3.0%	0.9%	0.6%	0.3%	0.2%	0.0X	4.6X	97.4%	34.8	35.7	0.02%	1.19	17.4	83.9%	16964	1.3%	67.2%	14.4	28.0%	0.19%	7.0	34.8	97.4%	97.4%	1.8%	35.7	89.5%	16.0	66.4%	48%	51 bp	20%	17.9
SRR12544536	42.1	41.1	49%	271	3.0%	0.9%	0.6%	0.2%	0.2%	0.0X	4.5X	97.3%	34.2	35.1	0.03%	1.18	17.1	83.9%	16934	1.2%	67.2%	14.1	27.8%	0.20%	6.9	34.2	97.3%	97.3%	1.8%	35.1	89.4%	15.7	66.2%	48%	51 bp	20%	17.6
SRR12544537	44.8	44.1	50%	235	2.7%	0.8%	0.5%	0.2%	0.1%	0.0X	4.2X	98.2%	35.7	36.4	0.03%	1.20	17.8	85.0%	16017	1.5%	66.1%	14.8	32.4%	0.18%	8.4	35.7	98.2%	98.2%	2.4%	36.4	90.2%	16.4	69.9%	49%	51 bp	20%	18.2
SRR12544538	44.0	43.4	50%	235	2.7%	0.8%	0.5%	0.2%	0.1%	0.0X	4.2X	98.2%	35.1	35.8	0.03%	1.21	17.6	85.0%	15981	1.5%	66.1%	14.6	32.2%	0.20%	8.3	35.1	98.2%	98.2%	2.4%	35.8	90.2%	16.1	69.6%	49%	51 bp	20%	17.9
SRR12544539	45.9	44.6	49%	279	3.4%	1.1%	0.7%	0.3%	0.2%	0.0X	4.8X	96.7%	36.8	38.1	0.02%	1.17	18.4	83.2%	17872	1.7%	65.6%	15.1	27.9%	0.18%	7.8	36.8	96.7%	96.7%	1.7%	38.1	88.0%	16.8	64.7%	48%	51 bp	20%	19.1
SRR12544540	45.1	43.9	49%	280	3.4%	1.0%	0.7%	0.3%	0.2%	0.0X	4.7X	96.6%	36.3	37.5	0.03%	1.17	18.1	83.2%	17862	1.7%	65.6%	14.8	27.8%	0.20%	7.6	36.3	96.6%	96.6%	1.7%	37.5	88.0%	16.5	64.4%	48%	51 bp	20%	18.8
SRR12544541	47.5	46.7	49%	280	2.8%	0.9%	0.6%	0.3%	0.2%	0.0X	5.4X	98.1%	38.3	39.0	0.02%	1.22	19.2	86.5%	16491	2.5%	67.7%	16.1	30.5%	0.19%	8.4	38.3	98.1%	98.1%	2.1%	39.0	90.1%	17.6	69.4%	48%	51 bp	20%	19.5
SRR12544542	46.7	46.0	49%	280	2.8%	0.9%	0.6%	0.3%	0.2%	0.0X	5.3X	98.1%	37.7	38.4	0.02%	1.18	18.9	86.5%	16394	2.5%	67.8%	15.8	30.3%	0.21%	8.3	37.7	98.1%	98.1%	2.1%	38.4	90.1%	17.3	69.0%	48%	51 bp	20%	19.2
SRR12544543	49.5	48.7	50%	278	2.9%	1.0%	0.6%	0.3%	0.2%	0.0X	5.5X	98.0%	41.1	41.9	0.02%	1.19	20.5	86.0%	17095	1.8%	69.6%	17.2	28.6%	0.20%	7.6	41.1	98.0%	98.0%	1.6%	41.9	90.5%	18.9	68.7%	48%	51 bp	20%	20.9
SRR12544544	48.8	48.0	50%	279	2.9%	0.9%	0.6%	0.3%	0.2%	0.0X	5.4X	98.0%	40.5	41.3	0.03%	1.22	20.2	85.9%	17057	1.8%	69.6%	17.0	28.5%	0.22%	7.5	40.5	98.0%	98.0%	1.6%	41.3	90.4%	18.7	68.5%	48%	51 bp	20%	20.7
SRR12544545	42.8	41.4	49%	290	2.5%	0.8%	0.5%	0.2%	0.2%	0.0X	5.3X	96.2%	35.8	37.2	0.02%	1.21	17.9	85.2%	15332	1.9%	71.7%	15.4	27.8%	0.18%	5.6	35.8	96.2%	96.2%	1.4%	37.2	90.0%	16.7	69.5%	47%	51 bp	20%	18.6
SRR12544546	42.2	40.8	49%	290	2.5%	0.8%	0.5%	0.2%	0.2%	0.0X	5.2X	96.2%	35.2	36.6	0.02%	1.21	17.6	85.1%	15374	1.9%	71.7%	15.1	27.7%	0.20%	5.6	35.2	96.2%	96.2%	1.4%	36.6	90.0%	16.5	69.1%	47%	51 bp	20%	18.3
SRR12544547	45.9	44.9	50%	250	2.5%	0.8%	0.5%	0.2%	0.2%	0.0X	5.1X	97.4%	38.3	39.3	0.02%	1.23	19.1	85.7%	16064	2.1%	70.4%	16.2	28.9%	0.20%	6.6	38.3	97.4%	97.4%	1.4%	39.3	90.3%	17.7	70.5%	49%	51 bp	20%	19.6
SRR12544548	45.1	44.1	50%	250	2.5%	0.8%	0.5%	0.2%	0.2%	0.0X	5.1X	97.4%	37.6	38.6	0.03%	1.24	18.8	85.7%	16046	2.1%	70.4%	15.9	28.7%	0.21%	6.5	37.6	97.4%	97.4%	1.4%	38.6	90.3%	17.4	70.4%	49%	51 bp	20%	19.3
SRR12544549	47.0	46.0	48%	278	2.7%	0.9%	0.5%	0.3%	0.2%	0.0X	5.7X	97.4%	39.5	40.5	0.02%	1.21	19.7	84.4%	15260	1.8%	71.1%	16.7	28.0%	0.20%	6.5	39.5	97.4%	97.4%	1.5%	40.5	91.2%	18.5	70.0%	47%	51 bp	20%	20.3
SRR12544550	46.4	45.3	48%	278	2.7%	0.8%	0.5%	0.3%	0.2%	0.0X	5.6X	97.4%	38.9	40.0	0.02%	1.19	19.5	84.4%	15302	1.8%	71.1%	16.5	27.9%	0.21%	6.4	38.9	97.4%	97.4%	1.5%	40.0	91.2%	18.2	69.7%	47%	51 bp	20%	20.0
SRR12544551	40.8	40.3	50%	256	2.7%	0.9%	0.6%	0.3%	0.2%	0.0X	4.2X	98.4%	33.6	34.2	0.02%	1.23	16.8	86.3%	17042	1.6%	68.1%	13.9	26.9%	0.19%	6.6	33.6	98.4%	98.4%	1.5%	34.2	89.7%	15.3	66.2%	49%	51 bp	20%	17.1
SRR12544552	40.1	39.6	50%	256	2.7%	0.9%	0.6%	0.2%	0.2%	0.0X	4.2X	98.4%	33.1	33.6	0.03%	1.22	16.5	86.2%	17028	1.6%	68.1%	13.7	26.7%	0.21%	6.5	33.1	98.4%	98.4%	1.5%	33.6	89.7%	15.1	65.7%	49%	51 bp	20%	16.8
SRR12544553	46.7	46.1	50%	228	2.4%	0.8%	0.5%	0.2%	0.2%	0.0X	4.7X	98.4%	38.5	39.1	0.02%	1.18	19.3	87.2%	16262	1.8%	69.6%	16.3	31.1%	0.17%	7.6	38.5	98.4%	98.4%	1.8%	39.1	90.7%	17.7	71.3%	49%	51 bp	20%	19.6
SRR12544554	45.9	45.3	50%	228	2.4%	0.8%	0.5%	0.2%	0.2%	0.0X	4.6X	98.4%	37.8	38.5	0.02%	1.19	18.9	87.2%	16220	1.8%	69.6%	16.0	30.9%	0.18%	7.5	37.8	98.4%	98.4%	1.8%	38.5	90.7%	17.4	71.1%	49%	51 bp	20%	19.2
SRR12544555	46.0	44.8	49%	236	3.4%	1.0%	0.7%	0.3%	0.2%	0.0X	5.5X	97.0%	37.3	38.5	0.02%	1.20	18.7	82.6%	17209	1.2%	67.0%	15.4	23.2%	0.19%	7.5	37.3	97.0%	97.0%	2.0%	38.5	90.0%	17.3	63.7%	48%	51 bp	20%	19.2
SRR12544556	45.3	44.1	49%	237	3.4%	1.0%	0.7%	0.3%	0.2%	0.0X	5.4X	96.9%	36.7	37.9	0.02%	1.19	18.3	82.5%	17127	1.2%	66.9%	15.1	23.1%	0.21%	7.4	36.7	96.9%	96.9%	2.0%	37.9	89.9%	17.0	63.7%	48%	51 bp	20%	18.9
SRR12544557	47.8	47.4	50%	264	2.4%	0.8%	0.5%	0.2%	0.2%	0.0X	4.8X	98.9%	39.3	39.7	0.02%	1.24	19.6	87.6%	15858	2.2%	69.5%	16.6	33.0%	0.17%	8.1	39.3	98.9%	98.9%	2.0%	39.7	91.2%	18.1	71.6%	49%	51 bp	20%	19.9
SRR12544558	47.1	46.7	50%	265	2.4%	0.8%	0.5%	0.2%	0.2%	0.0X	4.7X	98.9%	38.7	39.1	0.03%	1.22	19.3	87.6%	15843	2.2%	69.5%	16.4	32.9%	0.19%	8.0	38.7	98.9%	98.9%	2.0%	39.1	91.2%	17.8	71.5%	49%	51 bp	20%	19.6
SRR12544559	48.2	47.7	50%	241	2.6%	0.8%	0.5%	0.2%	0.2%	0.0X	5.2X	98.9%	40.6	41.1	0.02%	1.25	20.3	87.3%	15935	1.7%	71.7%	17.3	33.3%	0.18%	7.1	40.6	98.9%	98.9%	1.5%	41.1	92.0%	18.9	71.9%	49%	51 bp	20%	20.5
SRR12544560	47.4	46.9	50%	241	2.6%	0.8%	0.5%	0.2%	0.2%	0.0X	5.1X	98.9%	40.0	40.4	0.02%	1.25	20.0	87.3%	15918	1.7%	71.7%	17.0	33.1%	0.19%	7.0	40.0	98.9%	98.9%	1.5%	40.4	91.9%	18.6	71.7%	49%	51 bp	20%	20.2
SRR12544561	43.4	41.9	49%	266	2.8%	0.9%	0.6%	0.2%	0.2%	0.0X	5.1X	96.1%	36.7	38.2	0.03%	1.21	18.4	83.3%	16189	1.8%	71.6%	15.5	28.6%	0.22%	5.2	36.7	96.1%	96.1%	1.1%	38.2	90.2%	17.2	69.3%	48%	51 bp	20%	19.1
SRR12544562	42.8	41.3	49%	267	2.8%	0.9%	0.5%	0.2%	0.2%	0.0X	5.0X	96.1%	36.2	37.7	0.03%	1.21	18.1	83.3%	16233	1.8%	71.6%	15.3	28.5%	0.23%	5.1	36.2	96.1%	96.1%	1.1%	37.7	90.1%	17.0	69.0%	48%	51 bp	20%	18.8
SRR12544563	36.6	34.4	49%	244	2.0%	0.6%	0.4%	0.2%	0.1%	0.0X	2.7X	92.8%	28.5	30.7	0.03%	1.20	14.3	81.2%	16139	3.7%	64.2%	11.7	40.0%	0.19%	5.9	28.5	92.8%	92.8%	1.6%	30.7	84.5%	13.0	74.8%	48%	51 bp	20%	15.4
SRR12544564	36.0	33.9	49%	244	2.0%	0.6%	0.4%	0.2%	0.1%	0.0X	2.7X	92.8%	28.1	30.3	0.03%	1.17	14.0	81.1%	16143	3.7%	64.1%	11.5	39.8%	0.20%	5.8	28.1	92.8%	92.8%	1.6%	30.3	84.4%	12.8	74.1%	48%	51 bp	20%	15.1
SRR12544565	44.8	42.6	52%	253	1.9%	0.6%	0.4%	0.2%	0.1%	0.0X	3.0X	93.7%	32.6	34.8	0.04%	1.21	16.3	82.7%	16073	1.7%	58.4%	13.1	45.5%	0.22%	10.0	32.6	93.7%	93.7%	2.6%	34.8	82.3%	14.3	77.3%	50%	51 bp	20%	17.4
SRR12544566	44.1	41.9	52%	253	1.9%	0.6%	0.4%	0.2%	0.1%	0.0X	3.0X	93.6%	32.1	34.3	0.04%	1.19	16.0	82.7%	16019	1.7%	58.3%	12.9	45.2%	0.24%	9.9	32.1	93.6%	93.6%	2.6%	34.3	82.3%	14.1	77.1%	50%	51 bp	20%	17.1
SRR12544567	45.6	43.0	52%	268	2.2%	0.7%	0.5%	0.2%	0.1%	0.0X	3.9X	93.0%	35.1	37.7	0.03%	1.23	17.5	81.3%	16249	1.6%	63.6%	14.5	39.3%	0.23%	7.9	35.1	93.0%	93.0%	2.0%	37.7	84.8%	16.0	74.6%	51%	51 bp	20%	18.8
SRR12544568	44.9	42.3	52%	268	2.2%	0.7%	0.5%	0.2%	0.1%	0.0X	3.8X	93.0%	34.5	37.1	0.04%	1.21	17.3	81.3%	16245	1.6%	63.6%	14.3	39.1%	0.24%	7.8	34.5	93.0%	93.0%	2.0%	37.1	84.8%	15.7	74.5%	51%	51 bp	20%	18.6
SRR12544569	43.8	42.4	49%	251	3.1%	0.9%	0.6%	0.3%	0.2%	0.0X	4.4X	96.1%	35.1	36.5	0.03%	1.20	17.5	82.0%	17241	1.7%	65.3%	14.3	27.0%	0.20%	7.3	35.1	96.1%	96.1%	1.8%	36.5	87.9%	16.0	66.5%	48%	51 bp	20%	18.3
SRR12544570	43.1	41.7	49%	251	3.1%	0.9%	0.6%	0.3%	0.2%	0.0X	4.4X	96.1%	34.5	35.9	0.03%	1.21	17.3	82.0%	17211	1.7%	65.3%	14.1	26.8%	0.22%	7.1	34.5	96.1%	96.1%	1.8%	35.9	87.9%	15.8	66.1%	48%	51 bp	20%	18.0
SRR12544571	40.9	39.2	52%	206	1.5%	0.5%	0.3%	0.1%	0.1%	0.0X	2.4X	94.1%	27.3	28.9	0.04%	1.15	13.6	80.8%	15335	1.5%	51.4%	10.5	49.1%	0.20%	11.9	27.3	94.1%	94.1%	4.6%	28.9	80.8%	11.7	79.1%	51%	51 bp	20%	14.5
SRR12544572	40.1	38.4	52%	206	1.5%	0.5%	0.3%	0.1%	0.1%	0.0X	2.4X	94.1%	26.7	28.4	0.04%	1.19	13.4	80.8%	15322	1.5%	51.4%	10.3	48.6%	0.22%	11.7	26.7	94.1%	94.1%	4.6%	28.4	80.7%	11.5	78.3%	51%	51 bp	20%	14.2
SRR12544573	39.8	38.5	51%	237	1.5%	0.5%	0.3%	0.1%	0.1%	0.0X	3.0X	95.9%	30.0	31.3	0.03%	1.23	15.0	85.8%	14351	2.6%	63.6%	12.6	49.7%	0.17%	8.5	30.0	95.9%	95.9%	3.0%	31.3	86.9%	13.6	79.5%	50%	51 bp	20%	15.7
SRR12544574	39.1	37.8	51%	238	1.5%	0.5%	0.3%	0.1%	0.1%	0.0X	2.9X	95.8%	29.5	30.8	0.03%	1.23	14.8	85.7%	14333	2.6%	63.6%	12.4	49.4%	0.19%	8.3	29.5	95.8%	95.8%	3.0%	30.8	86.9%	13.4	79.0%	50%	51 bp	20%	15.4
SRR12544575	36.9	33.6	48%	251	3.7%	1.0%	0.6%	0.2%	0.1%	0.0X	4.4X	89.8%	29.5	32.8	0.02%	1.17	14.7	73.2%	16658	0.6%	63.8%	11.8	20.9%	0.20%	4.1	29.5	89.8%	89.8%	0.9%	32.8	84.2%	13.8	59.4%	47%	51 bp	20%	16.4
SRR12544576	36.3	33.0	48%	251	3.6%	1.0%	0.6%	0.2%	0.1%	0.0X	4.3X	89.8%	29.0	32.3	0.02%	1.19	14.5	73.1%	16653	0.6%	63.8%	11.6	20.8%	0.22%	4.1	29.0	89.8%	89.8%	0.9%	32.3	84.2%	13.6	59.0%	47%	51 bp	20%	16.1
SRR12544577	41.1	39.5	49%	283	3.6%	1.1%	0.7%	0.3%	0.2%	0.0X	5.5X	95.5%	34.1	35.7	0.02%	1.17	17.1	82.0%	18109	1.0%	68.1%	14.0	24.0%	0.20%	5.3	34.1	95.5%	95.5%	1.0%	35.7	88.2%	15.8	59.2%	48%	51 bp	20%	17.9
SRR12544578	40.4	38.8	49%	283	3.6%	1.1%	0.7%	0.3%	0.2%	0.0X	5.4X	95.5%	33.6	35.1	0.02%	1.15	16.8	82.0%	18007	1.0%	68.0%	13.8	23.8%	0.21%	5.3	33.6	95.5%	95.5%	1.0%	35.1	88.2%	15.5	58.8%	48%	51 bp	20%	17.6
SRR12544579	37.4	35.9	53%	219	1.6%	0.5%	0.3%	0.2%	0.1%	0.0X	2.8X	95.2%	28.5	29.9	0.03%	1.26	14.2	84.1%	15614	1.1%	61.1%	11.4	44.7%	0.21%	7.4	28.5	95.2%	95.2%	2.1%	29.9	84.6%	12.7	75.5%	51%	51 bp	10%	15.0
SRR12544580	36.7	35.2	53%	220	1.6%	0.5%	0.3%	0.2%	0.1%	0.0X	2.8X	95.1%	27.9	29.4	0.04%	1.24	14.0	84.1%	15603	1.0%	61.0%	11.2	44.3%	0.22%	7.3	27.9	95.1%	95.1%	2.1%	29.4	84.5%	12.4	75.1%	51%	51 bp	10%	14.7
SRR12544581	41.2	39.6	48%	255	3.8%	1.1%	0.7%	0.3%	0.2%	0.0X	5.3X	95.6%	35.0	36.6	0.02%	1.12	17.5	80.5%	17448	2.0%	69.4%	14.3	23.2%	0.18%	4.6	35.0	95.6%	95.6%	0.8%	36.6	89.2%	16.3	59.8%	47%	51 bp	20%	18.3
SRR12544582	40.6	39.0	48%	256	3.8%	1.1%	0.7%	0.3%	0.2%	0.0X	5.2X	95.6%	34.4	36.0	0.02%	1.12	17.2	80.4%	17421	2.0%	69.3%	14.1	23.1%	0.20%	4.6	34.4	95.6%	95.6%	0.8%	36.0	89.1%	16.0	59.4%	47%	51 bp	20%	18.0
SRR12544583	39.1	37.7	49%	262	2.7%	0.9%	0.6%	0.2%	0.1%	0.0X	4.2X	95.6%	31.9	33.4	0.02%	1.18	15.9	84.5%	17156	1.7%	67.1%	13.1	29.5%	0.19%	5.8	31.9	95.6%	95.6%	1.1%	33.4	86.7%	14.5	65.2%	48%	51 bp	20%	16.7
SRR12544584	38.5	37.0	49%	263	2.7%	0.9%	0.5%	0.2%	0.1%	0.0X	4.1X	95.6%	31.3	32.8	0.03%	1.18	15.7	84.4%	17127	1.7%	67.0%	12.9	29.2%	0.21%	5.7	31.3	95.6%	95.6%	1.1%	32.8	86.6%	14.2	64.7%	48%	51 bp	20%	16.4
SRR12544585	38.8	37.1	47%	256	3.4%	1.0%	0.7%	0.3%	0.2%	0.0X	4.9X	95.2%	32.8	34.4	0.02%	1.17	16.4	81.9%	17523	0.8%	70.3%	13.6	21.7%	0.19%	4.4	32.8	95.2%	95.2%	0.8%	34.4	88.9%	15.3	59.7%	46%	51 bp	20%	17.2
SRR12544586	38.2	36.5	47%	257	3.4%	1.0%	0.7%	0.3%	0.2%	0.0X	4.9X	95.2%	32.2	33.9	0.02%	1.15	16.1	81.8%	17474	0.8%	70.3%	13.4	21.5%	0.21%	4.3	32.2	95.2%	95.2%	0.8%	33.9	88.9%	15.1	59.4%	46%	51 bp	20%	16.9
SRR12544587	39.7	37.1	49%	236	2.7%	0.8%	0.5%	0.2%	0.1%	0.0X	3.7X	92.4%	32.1	34.7	0.03%	1.23	16.0	81.5%	16767	2.5%	67.9%	13.5	32.5%	0.21%	5.0	32.1	92.4%	92.4%	1.0%	34.7	85.5%	14.8	67.1%	48%	51 bp	20%	17.4
SRR12544588	39.0	36.4	49%	237	2.7%	0.8%	0.5%	0.2%	0.1%	0.0X	3.7X	92.3%	31.5	34.1	0.03%	1.23	15.8	81.4%	16752	2.5%	67.8%	13.2	32.3%	0.23%	4.9	31.5	92.3%	92.3%	1.0%	34.1	85.4%	14.6	66.5%	48%	51 bp	20%	17.1
SRR12544589	37.8	36.3	47%	260	3.2%	1.0%	0.6%	0.3%	0.2%	0.0X	4.9X	95.3%	31.9	33.4	0.02%	1.14	15.9	81.7%	16975	0.8%	70.3%	13.3	22.6%	0.19%	4.4	31.9	95.3%	95.3%	1.1%	33.4	89.3%	14.9	60.9%	46%	51 bp	20%	16.7
SRR12544590	37.3	35.7	47%	260	3.2%	1.0%	0.6%	0.3%	0.2%	0.0X	4.8X	95.2%	31.4	33.0	0.02%	1.17	15.7	81.6%	16944	0.8%	70.3%	13.1	22.4%	0.21%	4.3	31.4	95.2%	95.2%	1.1%	33.0	89.3%	14.7	60.6%	46%	51 bp	20%	16.5
SRR12544591	37.7	35.0	49%	237	3.1%	0.9%	0.5%	0.2%	0.1%	0.0X	4.2X	92.0%	30.9	33.6	0.02%	1.17	15.5	77.7%	15943	2.0%	67.9%	12.8	24.0%	0.20%	4.1	30.9	92.0%	92.0%	0.9%	33.6	86.5%	14.5	63.1%	48%	51 bp	20%	16.8
SRR12544592	37.0	34.3	49%	238	3.1%	0.9%	0.5%	0.2%	0.1%	0.0X	4.2X	92.0%	30.3	33.0	0.03%	1.15	15.2	77.7%	15842	2.0%	67.9%	12.6	23.8%	0.21%	4.0	30.3	92.0%	92.0%	0.9%	33.0	86.5%	14.3	62.5%	48%	51 bp	20%	16.5
SRR12544593	42.2	40.2	48%	250	3.4%	1.1%	0.7%	0.3%	0.2%	0.0X	5.6X	94.7%	36.1	38.1	0.02%	1.18	18.0	80.6%	17153	0.7%	71.2%	15.0	21.3%	0.20%	4.1	36.1	94.7%	94.7%	0.7%	38.1	89.4%	17.0	61.4%	47%	51 bp	20%	19.0
SRR12544594	41.5	39.5	48%	250	3.4%	1.0%	0.7%	0.3%	0.2%	0.0X	5.5X	94.7%	35.5	37.5	0.02%	1.17	17.7	80.5%	17146	0.7%	71.2%	14.8	21.1%	0.22%	4.0	35.5	94.7%	94.7%	0.7%	37.5	89.3%	16.7	61.0%	47%	51 bp	20%	18.7
SRR12544595	35.6	32.5	50%	231	2.3%	0.7%	0.5%	0.2%	0.1%	0.0X	3.3X	90.2%	28.4	31.5	0.03%	1.21	14.2	78.7%	16699	1.0%	66.6%	11.9	28.3%	0.24%	4.2	28.4	90.2%	90.2%	1.0%	31.5	83.9%	13.2	66.4%	49%	51 bp	20%	15.7
SRR12544596	35.0	31.9	50%	232	2.3%	0.7%	0.5%	0.2%	0.1%	0.0X	3.2X	90.1%	27.8	30.9	0.04%	1.17	13.9	78.6%	16667	1.0%	66.6%	11.6	28.1%	0.25%	4.1	27.8	90.1%	90.1%	1.0%	30.9	83.9%	13.0	66.1%	49%	51 bp	20%	15.5
SRR12544597	41.0	39.3	51%	225	2.1%	0.7%	0.4%	0.2%	0.1%	0.0X	4.9X	95.0%	32.3	34.0	0.03%	1.21	16.2	84.2%	16498	1.0%	63.5%	13.0	34.1%	0.26%	7.0	32.3	95.0%	95.0%	1.5%	34.0	85.2%	14.5	70.2%	50%	51 bp	30%	17.0
SRR12544598	40.3	38.6	51%	226	2.1%	0.7%	0.4%	0.2%	0.1%	0.0X	4.9X	94.9%	31.8	33.5	0.03%	1.22	15.9	84.1%	16470	1.0%	63.4%	12.8	33.8%	0.28%	6.9	31.8	94.9%	94.9%	1.5%	33.5	85.1%	14.2	69.8%	50%	51 bp	30%	16.7
SRR12544599	39.8	38.1	52%	214	1.8%	0.6%	0.4%	0.2%	0.1%	0.0X	3.1X	94.7%	29.1	30.7	0.04%	1.27	14.5	84.2%	15846	0.9%	58.3%	11.6	38.9%	0.23%	9.0	29.1	94.7%	94.7%	3.1%	30.7	83.2%	12.8	72.8%	51%	51 bp	20%	15.4
SRR12544600	39.0	37.4	52%	215	1.7%	0.5%	0.4%	0.2%	0.1%	0.0X	3.0X	94.6%	28.5	30.1	0.04%	1.24	14.3	84.2%	15834	0.9%	58.3%	11.4	38.6%	0.24%	8.9	28.5	94.6%	94.6%	3.1%	30.1	83.2%	12.5	72.5%	51%	51 bp	10%	15.1
SRR12544601	33.9	32.4	48%	224	3.4%	1.0%	0.6%	0.2%	0.1%	0.0X	4.2X	94.8%	28.1	29.6	0.02%	1.19	14.0	80.4%	17139	1.4%	67.6%	11.5	21.9%	0.21%	4.3	28.1	94.8%	94.8%	1.1%	29.6	88.1%	13.0	56.6%	47%	51 bp	20%	14.8
SRR12544602	33.3	31.8	48%	225	3.4%	1.0%	0.6%	0.2%	0.1%	0.0X	4.1X	94.7%	27.6	29.1	0.02%	1.20	13.8	80.4%	17109	1.4%	67.6%	11.3	21.7%	0.22%	4.2	27.6	94.7%	94.7%	1.1%	29.1	88.0%	12.8	56.0%	47%	51 bp	20%	14.6
SRR12544603	38.4	36.5	48%	243	3.2%	1.0%	0.7%	0.3%	0.2%	0.0X	4.9X	94.5%	32.7	34.6	0.02%	1.14	16.3	81.0%	17019	1.1%	71.5%	13.7	21.1%	0.20%	3.8	32.7	94.5%	94.5%	0.8%	34.6	89.2%	15.4	60.2%	47%	51 bp	20%	17.3
SRR12544604	37.8	35.9	48%	244	3.2%	1.0%	0.6%	0.3%	0.2%	0.0X	4.8X	94.5%	32.2	34.0	0.02%	1.17	16.1	81.0%	17050	1.1%	71.5%	13.5	21.0%	0.21%	3.7	32.2	94.5%	94.5%	0.8%	34.0	89.2%	15.2	60.0%	47%	51 bp	20%	17.0
SRR12544605	37.9	34.0	48%	245	2.7%	0.8%	0.5%	0.2%	0.1%	0.0X	4.1X	88.3%	29.3	33.2	0.02%	1.16	14.7	76.3%	16010	1.7%	64.7%	12.3	27.9%	0.21%	4.6	29.3	88.3%	88.3%	1.2%	33.2	82.1%	13.6	66.6%	47%	51 bp	20%	16.6
SRR12544606	37.3	33.4	48%	245	2.7%	0.8%	0.5%	0.2%	0.1%	0.0X	4.0X	88.2%	28.8	32.7	0.03%	1.16	14.4	76.3%	15948	1.7%	64.7%	12.1	27.7%	0.22%	4.6	28.8	88.2%	88.2%	1.2%	32.7	82.1%	13.4	66.2%	47%	51 bp	20%	16.3
SRR12544607	37.9	33.7	50%	245	2.4%	0.8%	0.5%	0.2%	0.1%	0.0X	3.4X	87.0%	28.1	32.3	0.03%	1.23	14.0	75.9%	16957	1.0%	61.4%	11.6	26.8%	0.21%	5.6	28.1	87.0%	87.0%	1.6%	32.3	79.8%	12.9	65.5%	49%	51 bp	20%	16.1
SRR12544608	37.2	33.1	50%	245	2.4%	0.8%	0.5%	0.2%	0.1%	0.0X	3.3X	86.9%	27.6	31.7	0.03%	1.25	13.8	75.8%	16947	1.0%	61.3%	11.4	26.6%	0.23%	5.5	27.6	86.9%	86.9%	1.5%	31.7	79.7%	12.7	65.1%	49%	51 bp	20%	15.9
SRR12544609	35.8	33.7	48%	282	3.7%	1.1%	0.7%	0.3%	0.2%	0.0X	4.7X	93.6%	30.3	32.3	0.02%	1.17	15.1	78.2%	17355	1.4%	69.8%	12.5	18.1%	0.17%	3.5	30.3	93.6%	93.6%	0.7%	32.3	88.3%	14.3	55.5%	47%	51 bp	20%	16.2
SRR12544610	35.2	33.2	48%	283	3.7%	1.1%	0.7%	0.3%	0.2%	0.0X	4.7X	93.5%	29.8	31.9	0.02%	1.14	14.9	78.1%	17400	1.4%	69.8%	12.3	18.0%	0.19%	3.4	29.8	93.5%	93.5%	0.7%	31.9	88.3%	14.1	55.0%	47%	51 bp	20%	15.9
SRR12544611	39.9	38.0	50%	274	3.1%	1.0%	0.6%	0.2%	0.1%	0.0X	4.6X	94.3%	32.3	34.2	0.03%	1.19	16.1	81.3%	16841	1.4%	66.5%	13.3	28.1%	0.24%	5.7	32.3	94.3%	94.3%	1.4%	34.2	86.8%	14.8	62.2%	49%	51 bp	20%	17.1
SRR12544612	39.4	37.4	50%	275	3.1%	0.9%	0.6%	0.2%	0.1%	0.0X	4.5X	94.3%	31.8	33.7	0.03%	1.20	15.9	81.3%	16861	1.4%	66.5%	13.1	28.0%	0.25%	5.6	31.8	94.3%	94.3%	1.4%	33.7	86.7%	14.6	61.9%	49%	51 bp	20%	16.9
SRR12544613	37.7	34.9	47%	265	3.2%	1.0%	0.6%	0.3%	0.2%	0.0X	5.0X	91.7%	31.3	34.1	0.02%	1.15	15.7	77.6%	16336	0.6%	69.1%	13.0	21.1%	0.19%	3.6	31.3	91.7%	91.7%	0.8%	34.1	86.7%	14.8	61.1%	46%	51 bp	20%	17.1
SRR12544614	37.2	34.4	47%	265	3.1%	1.0%	0.6%	0.3%	0.2%	0.0X	4.9X	91.6%	30.8	33.6	0.02%	1.17	15.4	77.6%	16349	0.6%	69.1%	12.8	21.0%	0.21%	3.5	30.8	91.6%	91.6%	0.8%	33.6	86.6%	14.6	60.8%	46%	51 bp	20%	16.8
SRR12544615	41.5	38.3	48%	312	2.3%	0.8%	0.5%	0.2%	0.1%	0.0X	5.9X	91.1%	33.3	36.6	0.02%	1.17	16.7	81.6%	14938	1.0%	69.4%	14.4	34.0%	0.18%	4.9	33.3	91.1%	91.1%	1.2%	36.6	85.3%	15.6	70.4%	47%	51 bp	20%	18.3
SRR12544616	40.9	37.7	48%	313	2.2%	0.8%	0.5%	0.2%	0.1%	0.0X	5.8X	91.0%	32.8	36.1	0.02%	1.16	16.4	81.6%	14903	1.0%	69.4%	14.2	33.8%	0.20%	4.9	32.8	91.0%	91.0%	1.2%	36.1	85.2%	15.4	70.3%	47%	51 bp	20%	18.0
SRR12544617	36.1	34.4	48%	242	2.5%	0.8%	0.5%	0.2%	0.1%	0.0X	3.8X	94.7%	30.6	32.3	0.03%	1.19	15.3	81.8%	15637	2.0%	71.5%	12.9	28.2%	0.21%	3.8	30.6	94.7%	94.7%	0.9%	32.3	89.1%	14.4	66.8%	47%	51 bp	20%	16.1
SRR12544618	35.5	33.8	48%	242	2.5%	0.8%	0.5%	0.2%	0.1%	0.0X	3.8X	94.6%	30.0	31.7	0.03%	1.19	15.0	81.8%	15513	2.0%	71.5%	12.7	28.1%	0.22%	3.8	30.0	94.6%	94.6%	0.9%	31.7	89.0%	14.1	66.7%	47%	51 bp	20%	15.9
SRR12544619	37.0	35.4	47%	256	3.0%	0.9%	0.6%	0.2%	0.2%	0.0X	4.7X	95.0%	31.2	32.9	0.02%	1.16	15.6	81.2%	16080	1.9%	70.3%	13.0	24.2%	0.17%	4.1	31.2	95.0%	95.0%	0.9%	32.9	89.1%	14.6	62.7%	46%	51 bp	20%	16.4
SRR12544620	36.5	34.8	47%	256	3.0%	0.9%	0.6%	0.2%	0.2%	0.0X	4.7X	95.0%	30.7	32.4	0.02%	1.18	15.4	81.2%	16085	1.9%	70.3%	12.8	24.0%	0.19%	4.1	30.7	95.0%	95.0%	1.0%	32.4	89.1%	14.4	62.4%	46%	51 bp	20%	16.2
SRR12544621	41.1	39.6	48%	263	3.1%	1.0%	0.6%	0.3%	0.2%	0.0X	4.6X	95.7%	34.0	35.5	0.02%	1.15	17.0	82.1%	16843	1.9%	68.6%	14.1	25.8%	0.19%	5.6	34.0	95.7%	95.7%	1.3%	35.5	88.8%	15.8	64.1%	47%	51 bp	20%	17.8
SRR12544622	40.5	39.0	48%	263	3.1%	0.9%	0.6%	0.3%	0.2%	0.0X	4.5X	95.7%	33.5	35.0	0.03%	1.12	16.7	82.0%	16791	1.9%	68.5%	13.9	25.7%	0.21%	5.5	33.5	95.7%	95.7%	1.3%	35.0	88.8%	15.5	63.6%	47%	51 bp	20%	17.5
SRR12544623	38.3	36.4	50%	240	2.2%	0.7%	0.4%	0.2%	0.1%	0.0X	3.4X	94.4%	30.9	32.8	0.03%	1.25	15.5	82.1%	15630	1.2%	68.1%	13.0	30.7%	0.18%	5.5	30.9	94.4%	94.4%	1.7%	32.8	87.8%	14.4	70.1%	48%	51 bp	20%	16.4
SRR12544624	37.7	35.8	50%	241	2.2%	0.7%	0.4%	0.2%	0.1%	0.0X	3.3X	94.3%	30.4	32.3	0.03%	1.25	15.2	82.0%	15682	1.2%	68.1%	12.8	30.5%	0.19%	5.4	30.4	94.3%	94.3%	1.6%	32.3	87.8%	14.2	69.6%	48%	51 bp	20%	16.1
SRR12544625	32.9	30.0	49%	268	2.9%	0.9%	0.5%	0.2%	0.1%	0.0X	3.7X	89.9%	25.6	28.5	0.02%	1.16	12.8	76.9%	16111	1.2%	63.9%	10.5	27.1%	0.21%	4.5	25.6	89.9%	89.9%	1.3%	28.5	83.0%	11.8	61.3%	48%	51 bp	30%	14.2
SRR12544626	32.4	29.6	49%	269	2.9%	0.9%	0.5%	0.2%	0.1%	0.0X	3.6X	89.8%	25.2	28.0	0.03%	1.17	12.6	76.8%	16061	1.2%	63.9%	10.4	26.9%	0.23%	4.4	25.2	89.8%	89.8%	1.3%	28.0	83.0%	11.6	60.8%	48%	51 bp	30%	14.0
SRR12544627	33.2	29.9	49%	238	2.6%	0.8%	0.5%	0.2%	0.1%	0.0X	3.4X	88.9%	26.0	29.3	0.03%	1.17	13.0	76.9%	16377	1.8%	65.6%	10.9	25.5%	0.21%	3.9	26.0	88.9%	88.9%	1.1%	29.3	82.9%	12.1	62.6%	48%	51 bp	20%	14.6
SRR12544628	32.6	29.4	49%	239	2.6%	0.8%	0.5%	0.2%	0.1%	0.0X	3.3X	88.8%	25.6	28.8	0.03%	1.16	12.8	76.8%	16353	1.8%	65.5%	10.7	25.2%	0.22%	3.8	25.6	88.8%	88.8%	1.1%	28.8	82.8%	11.9	62.4%	48%	51 bp	20%	14.4
SRR12544629	36.6	35.2	49%	237	2.5%	0.8%	0.5%	0.2%	0.1%	0.0X	4.0X	95.4%	29.8	31.2	0.02%	1.17	14.9	83.6%	16002	1.2%	68.6%	12.6	27.2%	0.19%	5.4	29.8	95.4%	95.4%	1.6%	31.2	88.5%	13.8	65.1%	48%	51 bp	20%	15.6
SRR12544630	36.0	34.6	49%	237	2.4%	0.8%	0.5%	0.2%	0.1%	0.0X	3.9X	95.4%	29.3	30.7	0.02%	1.16	14.6	83.6%	16023	1.2%	68.5%	12.3	27.0%	0.20%	5.3	29.3	95.4%	95.4%	1.6%	30.7	88.4%	13.6	64.6%	48%	51 bp	20%	15.4
SRR12544631	34.8	31.3	50%	233	1.7%	0.6%	0.4%	0.2%	0.1%	0.0X	2.6X	88.2%	26.3	29.8	0.04%	1.20	13.1	78.7%	15076	2.8%	63.2%	11.0	40.4%	0.23%	5.0	26.3	88.2%	88.2%	1.3%	29.8	80.5%	12.0	73.7%	49%	51 bp	30%	14.9
SRR12544632	34.3	30.8	50%	233	1.7%	0.6%	0.3%	0.2%	0.1%	0.0X	2.5X	88.1%	25.8	29.3	0.04%	1.19	12.9	78.6%	15038	2.8%	63.1%	10.8	40.1%	0.24%	5.0	25.8	88.1%	88.1%	1.3%	29.3	80.4%	11.8	73.1%	49%	51 bp	30%	14.7
SRR12544633	34.4	30.8	49%	254	2.6%	0.8%	0.5%	0.2%	0.1%	0.0X	3.5X	87.9%	26.5	30.2	0.03%	1.20	13.3	76.3%	16907	1.3%	63.8%	11.0	28.7%	0.22%	4.2	26.5	87.9%	87.9%	1.0%	30.2	81.2%	12.3	64.4%	48%	51 bp	20%	15.1
SRR12544634	33.9	30.2	49%	255	2.6%	0.8%	0.5%	0.2%	0.1%	0.0X	3.5X	87.8%	26.1	29.7	0.03%	1.21	13.0	76.2%	16898	1.4%	63.8%	10.8	28.4%	0.24%	4.2	26.1	87.8%	87.8%	1.0%	29.7	81.1%	12.0	64.2%	48%	51 bp	20%	14.8
SRR12544635	36.6	34.9	48%	231	2.9%	0.9%	0.6%	0.2%	0.1%	0.0X	3.8X	94.6%	29.6	31.3	0.03%	1.21	14.8	81.9%	17015	3.0%	66.2%	12.1	31.3%	0.20%	5.3	29.6	94.6%	94.6%	1.3%	31.3	86.7%	13.6	64.3%	47%	51 bp	20%	15.6
SRR12544636	36.0	34.3	48%	231	2.9%	0.9%	0.5%	0.2%	0.1%	0.0X	3.7X	94.6%	29.1	30.7	0.03%	1.16	14.5	81.8%	16959	3.0%	66.2%	11.9	31.0%	0.22%	5.2	29.1	94.6%	94.6%	1.3%	30.7	86.7%	13.3	64.0%	47%	51 bp	20%	15.4
SRR12544637	37.9	33.8	49%	276	3.5%	1.0%	0.6%	0.2%	0.1%	0.0X	4.2X	87.6%	29.3	33.5	0.03%	1.17	14.7	71.5%	16249	1.7%	62.1%	11.8	26.2%	0.22%	4.5	29.3	87.6%	87.6%	1.1%	33.5	81.8%	13.7	61.5%	48%	51 bp	30%	16.7
SRR12544638	37.3	33.2	49%	277	3.5%	1.0%	0.6%	0.2%	0.1%	0.0X	4.2X	87.5%	28.8	33.0	0.03%	1.12	14.4	71.4%	16197	1.7%	62.1%	11.6	26.1%	0.24%	4.4	28.8	87.5%	87.5%	1.1%	33.0	81.7%	13.5	61.2%	48%	51 bp	30%	16.5
SRR12544639	43.0	40.5	50%	271	3.1%	1.0%	0.6%	0.3%	0.2%	0.0X	4.9X	93.2%	33.9	36.3	0.02%	1.17	16.9	80.3%	17163	1.2%	64.2%	13.8	26.4%	0.20%	6.6	33.9	93.2%	93.2%	1.4%	36.3	85.0%	15.4	64.1%	49%	51 bp	20%	18.2
SRR12544640	42.3	39.9	50%	271	3.1%	1.0%	0.6%	0.2%	0.2%	0.0X	4.8X	93.2%	33.4	35.8	0.03%	1.21	16.7	80.2%	17144	1.2%	64.2%	13.6	26.2%	0.22%	6.5	33.4	93.2%	93.2%	1.4%	35.8	84.9%	15.2	63.9%	49%	51 bp	20%	17.9
SRR12544641	39.8	38.1	50%	253	2.6%	0.8%	0.5%	0.2%	0.1%	0.0X	3.6X	94.7%	32.0	33.8	0.03%	1.21	16.0	83.3%	17024	1.8%	66.3%	13.2	31.0%	0.20%	6.0	32.0	94.7%	94.7%	1.2%	33.8	86.1%	14.5	67.2%	49%	51 bp	30%	16.9
SRR12544642	39.2	37.4	50%	253	2.6%	0.8%	0.5%	0.2%	0.1%	0.0X	3.5X	94.7%	31.5	33.2	0.03%	1.18	15.7	83.3%	17003	1.8%	66.3%	13.0	30.8%	0.21%	5.9	31.5	94.7%	94.7%	1.2%	33.2	86.0%	14.3	66.7%	49%	51 bp	30%	16.6
SRR12544643	40.5	36.4	50%	250	2.8%	0.9%	0.5%	0.2%	0.1%	0.0X	4.1X	88.1%	30.5	34.6	0.02%	1.21	15.2	76.4%	16204	0.8%	62.0%	12.6	30.2%	0.19%	5.9	30.5	88.1%	88.1%	1.6%	34.6	81.0%	14.0	65.8%	49%	51 bp	20%	17.3
SRR12544644	39.8	35.8	50%	250	2.8%	0.8%	0.5%	0.2%	0.1%	0.0X	4.0X	88.1%	30.0	34.0	0.03%	1.18	15.0	76.3%	16183	0.8%	62.0%	12.4	30.0%	0.21%	5.8	30.0	88.1%	88.1%	1.6%	34.0	80.9%	13.8	65.5%	49%	51 bp	20%	17.0
SRR12544645	40.7	38.9	50%	246	2.7%	0.9%	0.6%	0.2%	0.1%	0.0X	4.4X	94.6%	32.0	33.8	0.02%	1.23	16.0	82.7%	17024	0.9%	65.0%	13.2	27.8%	0.20%	6.9	32.0	94.6%	94.6%	1.9%	33.8	86.5%	14.6	65.0%	49%	51 bp	20%	16.9
SRR12544646	40.1	38.3	50%	246	2.6%	0.9%	0.5%	0.2%	0.1%	0.0X	4.4X	94.6%	31.5	33.3	0.02%	1.21	15.7	82.7%	16972	0.9%	64.9%	13.0	27.6%	0.21%	6.8	31.5	94.6%	94.6%	1.9%	33.3	86.5%	14.4	64.8%	49%	51 bp	20%	16.6
SRR12544647	43.9	41.8	51%	250	2.6%	0.8%	0.5%	0.2%	0.2%	0.0X	4.1X	94.0%	34.0	36.2	0.03%	1.16	17.0	81.6%	17305	1.4%	62.9%	13.8	32.1%	0.21%	7.8	34.0	94.0%	94.0%	1.9%	36.2	85.0%	15.4	67.6%	50%	51 bp	20%	18.1
SRR12544648	43.2	41.1	51%	250	2.6%	0.8%	0.5%	0.2%	0.2%	0.0X	4.0X	94.0%	33.4	35.6	0.03%	1.16	16.7	81.5%	17300	1.4%	62.9%	13.6	31.9%	0.23%	7.6	33.4	94.0%	94.0%	1.9%	35.6	84.9%	15.1	67.3%	50%	51 bp	20%	17.8
SRR12544649	36.5	32.3	49%	270	3.3%	1.0%	0.6%	0.2%	0.1%	0.0X	4.2X	87.0%	27.9	32.0	0.02%	1.19	13.9	72.9%	16857	0.7%	62.5%	11.4	23.1%	0.21%	4.4	27.9	87.0%	87.0%	1.2%	32.0	81.2%	13.0	60.2%	48%	51 bp	20%	16.0
SRR12544650	35.9	31.8	49%	270	3.3%	0.9%	0.6%	0.2%	0.1%	0.0X	4.2X	87.0%	27.4	31.5	0.02%	1.20	13.7	72.8%	16768	0.7%	62.5%	11.2	23.0%	0.22%	4.4	27.4	87.0%	87.0%	1.2%	31.5	81.2%	12.8	60.0%	48%	51 bp	20%	15.8
SRR12544651	39.2	37.2	50%	202	1.5%	0.5%	0.3%	0.1%	0.1%	0.0X	2.2X	93.0%	26.5	28.5	0.04%	1.13	13.3	78.7%	14714	2.2%	52.4%	10.3	56.2%	0.21%	10.7	26.5	93.0%	93.0%	4.2%	28.5	80.9%	11.5	79.9%	49%	51 bp	10%	14.3
SRR12544652	38.5	36.5	50%	202	1.5%	0.5%	0.3%	0.1%	0.1%	0.0X	2.2X	93.0%	26.0	28.0	0.05%	1.12	13.0	78.7%	14712	2.3%	52.4%	10.1	55.7%	0.23%	10.5	26.0	93.0%	93.0%	4.2%	28.0	80.8%	11.3	79.4%	49%	51 bp	10%	14.0
SRR12544653	36.9	32.4	50%	240	2.3%	0.7%	0.4%	0.2%	0.1%	0.0X	3.3X	85.8%	27.4	32.0	0.03%	1.11	13.7	73.9%	15433	1.1%	61.9%	11.4	32.8%	0.23%	5.0	27.4	85.8%	85.8%	1.5%	32.0	79.8%	12.8	69.6%	49%	51 bp	20%	16.0
SRR12544654	36.3	31.8	50%	241	2.2%	0.7%	0.4%	0.2%	0.1%	0.0X	3.2X	85.7%	26.9	31.4	0.03%	1.10	13.5	73.9%	15467	1.1%	61.9%	11.2	32.5%	0.25%	4.9	26.9	85.7%	85.7%	1.5%	31.4	79.7%	12.5	69.2%	49%	51 bp	20%	15.7
SRR12544655	42.1	39.6	51%	263	2.4%	0.7%	0.5%	0.2%	0.1%	0.0X	3.8X	92.6%	31.4	33.9	0.03%	1.19	15.7	80.8%	16619	1.3%	60.4%	12.7	32.6%	0.22%	8.1	31.4	92.6%	92.6%	2.4%	33.9	83.0%	14.1	69.3%	50%	51 bp	20%	17.0
SRR12544656	41.5	39.0	51%	263	2.4%	0.7%	0.5%	0.2%	0.1%	0.0X	3.7X	92.5%	31.0	33.5	0.03%	1.18	15.5	80.8%	16598	1.3%	60.4%	12.5	32.4%	0.23%	8.0	31.0	92.5%	92.5%	2.4%	33.5	83.0%	13.9	68.7%	50%	51 bp	20%	16.7
SRR12544657	42.5	40.2	50%	247	1.9%	0.6%	0.4%	0.2%	0.1%	0.0X	3.6X	93.7%	34.3	36.6	0.03%	1.12	17.1	83.0%	15483	2.6%	68.1%	14.5	35.6%	0.19%	5.9	34.3	93.7%	93.7%	1.4%	36.6	86.7%	15.8	75.5%	49%	51 bp	20%	18.3
SRR12544658	41.8	39.5	50%	248	1.9%	0.6%	0.4%	0.2%	0.1%	0.0X	3.5X	93.7%	33.7	36.0	0.03%	1.14	16.9	82.9%	15555	2.6%	68.1%	14.2	35.4%	0.21%	5.8	33.7	93.7%	93.7%	1.4%	36.0	86.6%	15.6	75.3%	49%	51 bp	20%	18.0
SRR12544659	41.9	40.0	49%	301	2.4%	0.8%	0.5%	0.2%	0.1%	0.0X	4.1X	94.8%	34.5	36.4	0.03%	1.14	17.2	83.4%	16875	0.8%	69.0%	14.5	31.6%	0.19%	5.6	34.5	94.8%	94.8%	1.2%	36.4	87.7%	15.9	71.3%	48%	51 bp	20%	18.2
SRR12544660	41.3	39.5	49%	302	2.4%	0.8%	0.5%	0.2%	0.1%	0.0X	4.1X	94.8%	34.0	35.9	0.03%	1.12	17.0	83.3%	16796	0.8%	69.0%	14.3	31.4%	0.21%	5.5	34.0	94.8%	94.8%	1.2%	35.9	87.6%	15.7	70.9%	48%	51 bp	20%	17.9
SRR12544661	44.5	43.7	49%	269	2.9%	0.9%	0.6%	0.2%	0.2%	0.0X	4.2X	97.7%	35.9	36.8	0.03%	1.15	17.9	85.0%	17633	2.4%	65.6%	14.6	35.8%	0.20%	7.8	35.9	97.7%	97.7%	1.8%	36.8	88.3%	16.2	69.6%	48%	51 bp	20%	18.4
SRR12544662	43.9	43.0	49%	269	2.9%	0.9%	0.6%	0.2%	0.1%	0.0X	4.2X	97.6%	35.3	36.2	0.03%	1.19	17.7	85.0%	17575	2.4%	65.6%	14.4	35.6%	0.21%	7.7	35.3	97.6%	97.6%	1.8%	36.2	88.3%	16.0	69.2%	48%	51 bp	20%	18.1
SRR12544663	44.3	43.6	48%	283	3.1%	1.0%	0.6%	0.3%	0.2%	0.0X	6.6X	98.2%	38.3	39.0	0.02%	1.16	19.2	85.1%	16242	0.7%	73.8%	16.3	24.4%	0.17%	5.3	38.3	98.2%	98.2%	1.2%	39.0	92.8%	18.1	66.1%	47%	51 bp	20%	19.5
SRR12544664	43.7	43.0	48%	283	3.1%	1.0%	0.6%	0.3%	0.2%	0.0X	6.5X	98.2%	37.8	38.5	0.02%	1.13	18.9	85.0%	16221	0.7%	73.8%	16.1	24.3%	0.19%	5.2	37.8	98.2%	98.2%	1.2%	38.5	92.8%	17.8	65.8%	47%	51 bp	20%	19.2
SRR12544665	46.9	46.4	49%	267	2.5%	0.8%	0.5%	0.2%	0.2%	0.0X	5.4X	98.8%	40.6	41.1	0.02%	1.25	20.3	87.2%	16037	1.5%	74.0%	17.4	33.3%	0.19%	5.8	40.6	98.8%	98.8%	1.2%	41.1	92.6%	19.0	72.5%	48%	51 bp	20%	20.6
SRR12544666	46.3	45.8	49%	268	2.5%	0.8%	0.5%	0.2%	0.2%	0.0X	5.3X	98.7%	40.0	40.6	0.03%	1.26	20.0	87.1%	16069	1.5%	74.0%	17.1	33.1%	0.21%	5.7	40.0	98.7%	98.7%	1.2%	40.6	92.5%	18.8	72.3%	48%	51 bp	20%	20.3
SRR12544667	44.6	44.0	51%	225	2.0%	0.6%	0.4%	0.2%	0.1%	0.0X	2.9X	98.0%	32.4	33.1	0.03%	1.24	16.2	84.6%	16122	3.5%	57.7%	12.9	46.9%	0.19%	11.6	32.4	98.0%	98.0%	3.8%	33.1	86.2%	14.3	76.2%	50%	51 bp	20%	16.5
SRR12544668	43.9	43.2	51%	225	2.0%	0.6%	0.4%	0.2%	0.1%	0.0X	2.9X	98.0%	31.9	32.5	0.04%	1.22	15.9	84.5%	16069	3.5%	57.7%	12.7	46.6%	0.20%	11.4	31.9	98.0%	98.0%	3.8%	32.5	86.2%	14.0	75.5%	50%	51 bp	20%	16.3
SRR12544669	49.1	48.7	49%	259	2.9%	0.9%	0.6%	0.3%	0.2%	0.0X	5.0X	99.1%	39.9	40.3	0.02%	1.10	20.0	85.7%	16359	2.2%	67.7%	16.6	32.7%	0.17%	8.8	39.9	99.1%	99.1%	2.2%	40.3	91.2%	18.4	70.1%	48%	51 bp	20%	20.2
SRR12544670	48.4	48.0	49%	259	2.8%	0.9%	0.6%	0.3%	0.2%	0.0X	5.0X	99.1%	39.3	39.7	0.02%	1.14	19.7	85.7%	16317	2.2%	67.7%	16.4	32.5%	0.19%	8.6	39.3	99.1%	99.1%	2.2%	39.7	91.1%	18.1	69.7%	48%	51 bp	20%	19.9

Uncheck the tick box to hide columns. Click and drag the handle on the left to change order.

Sort	Group	Column	Description	ID	Scale
\|\|	Samtools	M Reads	Total reads in the bam file (millions)	`flagstat_total`	read_count
\|\|	Samtools	M Reads Mapped	Reads Mapped in the bam file (millions)	`mapped_passed`	read_count
\|\|	QualiMap	% GC	Mean GC content	`avg_gc`	None
\|\|	QualiMap	Ins. size	Median insert size	`median_insert_size`	None
\|\|	QualiMap	≥ 1X	Fraction of genome with at least 1X coverage	`1_x_pc`	None
\|\|	QualiMap	≥ 5X	Fraction of genome with at least 5X coverage	`5_x_pc`	None
\|\|	QualiMap	≥ 10X	Fraction of genome with at least 10X coverage	`10_x_pc`	None
\|\|	QualiMap	≥ 30X	Fraction of genome with at least 30X coverage	`30_x_pc`	None
\|\|	QualiMap	≥ 50X	Fraction of genome with at least 50X coverage	`50_x_pc`	None
\|\|	QualiMap	Median cov	Median coverage	`median_coverage`	None
\|\|	QualiMap	Mean cov	Mean coverage	`mean_coverage`	None
\|\|	QualiMap	% Aligned	% mapped reads	`percentage_aligned`	None
\|\|	QualiMap	M Aligned	Number of mapped reads (millions)	`mapped_reads`	read_count
\|\|	QualiMap	M Total reads	Number of reads (millions)	`total_reads`	read_count
\|\|	QualiMap	Error rate	Alignment error rate. Total edit distance (SAM NM field) over the number of mapped bases	`general_error_rate`	None
\|\|	QualiMap	5'-3' bias	5'-3' bias	`5_3_bias`	None
\|\|	QualiMap	M Aligned	Reads Aligned (millions)	`reads_aligned`	read_count
\|\|	RNA-SeQC	% Expression Efficiency	Ratio of exonic reads to total reads	`Expression Profiling Efficiency`	None
\|\|	RNA-SeQC	# Genes	Number of genes detected with at least 5 reads	`Genes Detected`	None
\|\|	RNA-SeQC	% rRNA Alignment	Ribosomal RNA reads per total reads	`rRNA rate`	None
\|\|	featureCounts	% Assigned	% Assigned reads	`percent_assigned`	None
\|\|	featureCounts	M Assigned	Assigned reads (millions)	`Assigned`	read_count
\|\|	Picard	% Dups	Mark Duplicates - Percent Duplication	`PERCENT_DUPLICATION`	None
\|\|	Samtools	Error rate	Error rate: mismatches (NM) / bases mapped (CIGAR)	`error_rate`	None
\|\|	Samtools	M Non-Primary	Non-primary alignments (millions)	`non-primary_alignments`	read_count
\|\|	Samtools	M Reads Mapped	Reads Mapped in the bam file (millions)	`reads_mapped`	read_count
\|\|	Samtools	% Mapped	% Mapped Reads	`reads_mapped_percent`	None
\|\|	Samtools	% Proper Pairs	% Properly Paired Reads	`reads_properly_paired_percent`	None
\|\|	Samtools	% MapQ 0 Reads	% of Reads that are Ambiguously Placed (MapQ=0)	`reads_MQ0_percent`	None
\|\|	Samtools	M Total seqs	Total sequences in the bam file (millions)	`raw_total_sequences`	read_count
\|\|	STAR	% Aligned	% Uniquely mapped reads	`uniquely_mapped_percent`	None
\|\|	STAR	M Aligned	Uniquely mapped reads (millions)	`uniquely_mapped`	read_count
\|\|	FastQC	% Dups	% Duplicate Reads	`percent_duplicates`	None
\|\|	FastQC	% GC	Average % GC Content	`percent_gc`	None
\|\|	FastQC	Length	Average Sequence Length (bp)	`avg_sequence_length`	None
\|\|	FastQC	% Failed	Percentage of modules failed in FastQC report (includes those not plotted here)	`percent_fails`	None
\|\|	FastQC	M Seqs	Total Sequences (millions)	`total_sequences`	read_count

QualiMap

QualiMap is a platform-independent application to facilitate the quality control of alignment sequencing data and its derivatives like feature counts.

Coverage histogram

Distribution of the number of locations in the reference genome with a given depth of coverage.

Bases of a reference sequence (y-axis) are groupped by their depth of coverage (0×, 1×, …, N×) (x-axis). This plot shows the frequency of coverage depths relative to the reference sequence for each read dataset, which provides an indirect measure of the level and variation of coverage depth in the corresponding sequenced sample.

If reads are randomly distributed across the reference sequence, this plot should resemble a Poisson distribution (Lander & Waterman 1988), with a peak indicating approximate depth of coverage, and more uniform coverage depth being reflected in a narrower spread. The optimal level of coverage depth depends on the aims of the experiment, though it should at minimum be sufficiently high to adequately address the biological question; greater uniformity of coverage is generally desirable, because it increases breadth of coverage for a given depth of coverage, allowing equivalent results to be achieved at a lower sequencing depth (Sampson et al. 2011; Sims et al. 2014). However, it is difficult to achieve uniform coverage depth in practice, due to biases introduced during sample preparation (van Dijk et al. 2014), sequencing (Ross et al. 2013) and read mapping (Sims et al. 2014).

This plot may include a small peak for regions of the reference sequence with zero depth of coverage. Such regions may be absent from the given sample (due to a deletion or structural rearrangement), present in the sample but not successfully sequenced (due to bias in sequencing or preparation), or sequenced but not successfully mapped to the reference (due to the choice of mapping algorithm, the presence of repeat sequences, or mismatches caused by variants or sequencing errors). Related factors cause most datasets to contain some unmapped reads (Sims et al. 2014).

Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).

Cumulative genome coverage

Percentage of the reference genome with at least the given depth of coverage.

For a set of DNA or RNA reads mapped to a reference sequence, such as a genome or transcriptome, the depth of coverage at a given base position is the number of high-quality reads that map to the reference at that position, while the breadth of coverage is the fraction of the reference sequence to which reads have been mapped with at least a given depth of coverage (Sims et al. 2014).

Defining coverage breadth in terms of coverage depth is useful, because sequencing experiments typically require a specific minimum depth of coverage over the region of interest (Sims et al. 2014), so the extent of the reference sequence that is amenable to analysis is constrained to lie within regions that have sufficient depth. With inadequate sequencing breadth, it can be difficult to distinguish the absence of a biological feature (such as a gene) from a lack of data (Green 2007).

For increasing coverage depths (1×, 2×, …, N×), coverage breadth is calculated as the percentage of the reference sequence that is covered by at least that number of reads, then plots coverage breadth (y-axis) against coverage depth (x-axis). This plot shows the relationship between sequencing depth and breadth for each read dataset, which can be used to gauge, for example, the likely effect of a minimum depth filter on the fraction of a genome available for analysis.

Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).

Insert size histogram

Distribution of estimated insert sizes of mapped reads.

To overcome limitations in the length of DNA or RNA sequencing reads, many sequencing instruments can produce two or more shorter reads from one longer fragment in which the relative position of reads is approximately known, such as paired-end or mate-pair reads (Mardis 2013). Such techniques can extend the reach of sequencing technology, allowing for more accurate placement of reads (Reinert et al. 2015) and better resolution of repeat regions (Reinert et al. 2015), as well as detection of structural variation (Alkan et al. 2011) and chimeric transcripts (Maher et al. 2009).

All these methods assume that the approximate size of an insert is known. (Insert size can be defined as the length in bases of a sequenced DNA or RNA fragment, excluding technical sequences such as adapters, which are typically removed before alignment.) This plot allows for that assumption to be assessed. With the set of mapped fragments for a given sample, QualiMap groups the fragments by insert size, then plots the frequency of mapped fragments (y-axis) over a range of insert sizes (x-axis). In an ideal case, the distribution of fragment sizes for a sequencing library would culminate in a single peak indicating average insert size, with a narrow spread indicating highly consistent fragment lengths.

QualiMap calculates insert sizes as follows: for each fragment in which every read mapped successfully to the same reference sequence, it extracts the insert size from the TLEN field of the leftmost read (see the Qualimap 2 documentation), where the TLEN (or 'observed Template LENgth') field contains 'the number of bases from the leftmost mapped base to the rightmost mapped base' (SAM format specification). Note that because it is defined in terms of alignment to a reference sequence, the value of the TLEN field may differ from the insert size due to factors such as alignment clipping, alignment errors, or structural variation or splicing in a gap between reads from the same fragment.

Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).

GC content distribution

Each solid line represents the distribution of GC content of mapped reads for a given sample.

GC bias is the difference between the guanine-cytosine content (GC-content) of a set of sequencing reads and the GC-content of the DNA or RNA in the original sample. It is a well-known issue with sequencing systems, and may be introduced by PCR amplification, among other factors (Benjamini & Speed 2012; Ross et al. 2013).

QualiMap calculates the GC-content of individual mapped reads, then groups those reads by their GC-content (1%, 2%, …, 100%), and plots the frequency of mapped reads (y-axis) at each level of GC-content (x-axis). This plot shows the GC-content distribution of mapped reads for each read dataset, which should ideally resemble that of the original sample. It can be useful to display the GC-content distribution of an appropriate reference sequence for comparison, and QualiMap has an option to do this (see the Qualimap 2 documentation).

Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).

Genomic origin of reads

Classification of mapped reads as originating in exonic, intronic or intergenic regions. These can be displayed as either the number or percentage of mapped reads.

There are currently three main approaches to map reads to transcripts in an RNA-seq experiment: mapping reads to a reference genome to identify expressed transcripts that are annotated (and discover those that are unknown), mapping reads to a reference transcriptome, and de novo assembly of transcript sequences (Conesa et al. 2016).

For RNA-seq QC analysis, QualiMap can be used to assess alignments produced by the first of these approaches. For input, it requires a GTF annotation file along with a reference genome, which can be used to reconstruct the exon structure of known transcripts. This allows mapped reads to be grouped by whether they originate in an exonic region (for QualiMap, this may include 5′ and 3′ UTR regions as well as protein-coding exons), an intron, or an intergenic region (see the Qualimap 2 documentation).

The inferred genomic origins of RNA-seq reads are presented here as a bar graph showing either the number or percentage of mapped reads in each read dataset that have been assigned to each type of genomic region. This graph can be used to assess the proportion of useful reads in an RNA-seq experiment. That proportion can be reduced by the presence of intron sequences, especially if depletion of ribosomal RNA was used during sample preparation (Sims et al. 2014). It can also be reduced by off-target transcripts, which are detected in greater numbers at the sequencing depths needed to detect poorly-expressed transcripts (Tarazona et al. 2011).

Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).

Gene Coverage Profile

Mean distribution of coverage depth across the length of all mapped transcripts.

For RNA-seq QC analysis, QualiMap can be used to assess alignments produced by the first of these approaches. For input, it requires a GTF annotation file along with a reference genome, which can be used to reconstruct the exon structure of known transcripts. QualiMap uses this information to calculate the depth of coverage along the length of each annotated transcript. For a set of reads mapped to a transcript, the depth of coverage at a given base position is the number of high-quality reads that map to the transcript at that position (Sims et al. 2014).

QualiMap calculates coverage depth at every base position of each annotated transcript. To enable meaningful comparison between transcripts, base positions are rescaled to relative positions expressed as percentage distance along each transcript (0%, 1%, …, 99%). For the set of transcripts with at least one mapped read, QualiMap plots the cumulative mapped-read depth (y-axis) at each relative transcript position (x-axis). This plot shows the gene coverage profile across all mapped transcripts for each read dataset. It provides a visual way to assess positional biases, such as an accumulation of mapped reads at the 3′ end of transcripts, which may indicate poor RNA quality in the original sample (Conesa et al. 2016).

Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).

RNA-SeQC

RNA-SeQC Fast, efficient RNA-Seq metrics for quality control and process optimization

Transcript-associated reads

All of the rates given are per mapped read.

Exonic Rate is the fraction mapping within exons.
Intronic Rate is the fraction mapping within introns.
Intergenic Rate is the fraction mapping in the genomic space between genes.

Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).

Strand Specificity

End 1/2 Sense are the number of End 1 or 2 reads that were sequenced in the sense direction.

End 1/2 Antisense are the number of End 1 or 2 reads that were sequenced in the antisense direction

Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).

Read Counts

Number of reads (millions) falling into different categories.

Note that many of these statistics are only available from RNA-SeQC v2.x

RSeQC

RSeQC package provides a number of useful modules that can comprehensively evaluate high throughput RNA-seq data.

Infer experiment

Infer experiment counts the percentage of reads and read pairs that match the strandedness of overlapping transcripts. It can be used to infer whether RNA-seq library preps are stranded (sense or antisense).

Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).

featureCounts

Subread featureCounts is a highly efficient general-purpose read summarization program that counts mapped reads for genomic features such as genes, exons, promoter, gene bodies, genomic bins and chromosomal locations.

Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).

Picard

Picard is a set of Java command line tools for manipulating high-throughput sequencing data.

Mark Duplicates

Number of reads, categorised by duplication state. Pair counts are doubled - see help text for details.

The table in the Picard metrics file contains some columns referring read pairs and some referring to single reads.

To make the numbers in this plot sum correctly, values referring to pairs are doubled according to the scheme below:

READS_IN_DUPLICATE_PAIRS = 2 * READ_PAIR_DUPLICATES
READS_IN_UNIQUE_PAIRS = 2 * (READ_PAIRS_EXAMINED - READ_PAIR_DUPLICATES)
READS_IN_UNIQUE_UNPAIRED = UNPAIRED_READS_EXAMINED - UNPAIRED_READ_DUPLICATES
READS_IN_DUPLICATE_PAIRS_OPTICAL = 2 * READ_PAIR_OPTICAL_DUPLICATES
READS_IN_DUPLICATE_PAIRS_NONOPTICAL = READS_IN_DUPLICATE_PAIRS - READS_IN_DUPLICATE_PAIRS_OPTICAL
READS_IN_DUPLICATE_UNPAIRED = UNPAIRED_READ_DUPLICATES
READS_UNMAPPED = UNMAPPED_READS

Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).

Samtools

Samtools is a suite of programs for interacting with high-throughput sequencing data.

Percent Mapped

Alignment metrics from samtools stats; mapped vs. unmapped reads.

For a set of samples that have come from the same multiplexed library, similar numbers of reads for each sample are expected. Large differences in numbers might indicate issues during the library preparation process. Whilst large differences in read numbers may be controlled for in downstream processings (e.g. read count normalisation), you may wish to consider whether the read depths achieved have fallen below recommended levels depending on the applications.

Low alignment rates could indicate contamination of samples (e.g. adapter sequences), low sequencing quality or other artefacts. These can be further investigated in the sequence level QC (e.g. from FastQC).

Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).

Alignment metrics

This module parses the output from samtools stats. All numbers in millions.

Samtools Flagstat

This module parses the output from samtools flagstat. All numbers in millions.

XY counts

Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).

Mapped reads per contig

The samtools idxstats tool counts the number of mapped reads per chromosome / contig. Chromosomes with < 0.1% of the total aligned reads are omitted from this plot.

Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).

STAR

STAR is an ultrafast universal RNA-seq aligner.

Alignment Scores

Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).

Gene Counts

Statistics from results generated using --quantMode GeneCounts. The three tabs show counts for unstranded RNA-seq, counts for the 1st read strand aligned with RNA and counts for the 2nd read strand aligned with RNA.

Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).

FastQC

FastQC is a quality control tool for high throughput sequence data, written by Simon Andrews at the Babraham Institute in Cambridge.

Sequence Counts

Sequence counts for each sample. Duplicate read counts are an estimate only.

This plot show the total number of reads, broken down into unique and duplicate if possible (only more recent versions of FastQC give duplicate info).

You can read more about duplicate calculation in the FastQC documentation. A small part has been copied here for convenience:

Only sequences which first appear in the first 100,000 sequences in each file are analysed. This should be enough to get a good impression for the duplication levels in the whole file. Each sequence is tracked to the end of the file to give a representative count of the overall duplication level.

The duplication detection requires an exact sequence match over the whole length of the sequence. Any reads over 75bp in length are truncated to 50bp for this analysis.

Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).

Sequence Quality Histograms

The mean quality value across each base position in the read.

To enable multiple samples to be plotted on the same graph, only the mean quality scores are plotted (unlike the box plots seen in FastQC reports).

Taken from the FastQC help:

The y-axis on the graph shows the quality scores. The higher the score, the better the base call. The background of the graph divides the y axis into very good quality calls (green), calls of reasonable quality (orange), and calls of poor quality (red). The quality of calls on most platforms will degrade as the run progresses, so it is common to see base calls falling into the orange area towards the end of a read.

Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).

Per Sequence Quality Scores

The number of reads with average quality scores. Shows if a subset of reads has poor quality.

From the FastQC help:

The per sequence quality score report allows you to see if a subset of your sequences have universally low quality values. It is often the case that a subset of sequences will have universally poor quality, however these should represent only a small percentage of the total sequences.

Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).

Per Base Sequence Content

The proportion of each base position for which each of the four normal DNA bases has been called.

To enable multiple samples to be shown in a single plot, the base composition data is shown as a heatmap. The colours represent the balance between the four bases: an even distribution should give an even muddy brown colour. Hover over the plot to see the percentage of the four bases under the cursor.

To see the data as a line plot, as in the original FastQC graph, click on a sample track.

From the FastQC help:

Per Base Sequence Content plots out the proportion of each base position in a file for which each of the four normal DNA bases has been called.

In a random library you would expect that there would be little to no difference between the different bases of a sequence run, so the lines in this plot should run parallel with each other. The relative amount of each base should reflect the overall amount of these bases in your genome, but in any case they should not be hugely imbalanced from each other.

It's worth noting that some types of library will always produce biased sequence composition, normally at the start of the read. Libraries produced by priming using random hexamers (including nearly all RNA-Seq libraries) and those which were fragmented using transposases inherit an intrinsic bias in the positions at which reads start. This bias does not concern an absolute sequence, but instead provides enrichement of a number of different K-mers at the 5' end of the reads. Whilst this is a true technical bias, it isn't something which can be corrected by trimming and in most cases doesn't seem to adversely affect the downstream analysis.

Click a sample row to see a line plot for that dataset.

Rollover for sample name

Position: -

%T: -

%C: -

%A: -

%G: -

Per Sequence GC Content

The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content.

From the FastQC help:

This module measures the GC content across the whole length of each sequence in a file and compares it to a modelled normal distribution of GC content.

In a normal random library you would expect to see a roughly normal distribution of GC content where the central peak corresponds to the overall GC content of the underlying genome. Since we don't know the the GC content of the genome the modal GC content is calculated from the observed data and used to build a reference distribution.

An unusually shaped distribution could indicate a contaminated library or some other kinds of biased subset. A normal distribution which is shifted indicates some systematic bias which is independent of base position. If there is a systematic bias which creates a shifted normal distribution then this won't be flagged as an error by the module since it doesn't know what your genome's GC content should be.

Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).

Per Base N Content

The percentage of base calls at each position for which an N was called.

From the FastQC help:

If a sequencer is unable to make a base call with sufficient confidence then it will normally substitute an N rather than a conventional base call. This graph shows the percentage of base calls at each position for which an N was called.

It's not unusual to see a very low proportion of Ns appearing in a sequence, especially nearer the end of a sequence. However, if this proportion rises above a few percent it suggests that the analysis pipeline was unable to interpret the data well enough to make valid base calls.

Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).

Sequence Length Distribution

All samples have sequences of a single length (51bp).

Sequence Duplication Levels

The relative level of duplication found for every sequence.

From the FastQC Help:

In a diverse library most sequences will occur only once in the final set. A low level of duplication may indicate a very high level of coverage of the target sequence, but a high level of duplication is more likely to indicate some kind of enrichment bias (eg PCR over amplification). This graph shows the degree of duplication for every sequence in a library: the relative number of sequences with different degrees of duplication.

The duplication detection requires an exact sequence match over the whole length of the sequence. Any reads over 75bp in length are truncated to 50bp for this analysis.

In a properly diverse library most sequences should fall into the far left of the plot in both the red and blue lines. A general level of enrichment, indicating broad oversequencing in the library will tend to flatten the lines, lowering the low end and generally raising other categories. More specific enrichments of subsets, or the presence of low complexity contaminants will tend to produce spikes towards the right of the plot.

Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).

Overrepresented sequences

The total amount of overrepresented sequences found in each library.

FastQC calculates and lists overrepresented sequences in FastQ files. It would not be possible to show this for all samples in a MultiQC report, so instead this plot shows the number of sequences categorized as over represented.

Sometimes, a single sequence may account for a large number of reads in a dataset. To show this, the bars are split into two: the first shows the overrepresented reads that come from the single most common sequence. The second shows the total count from all remaining overrepresented sequences.

From the FastQC Help:

A normal high-throughput library will contain a diverse set of sequences, with no individual sequence making up a tiny fraction of the whole. Finding that a single sequence is very overrepresented in the set either means that it is highly biologically significant, or indicates that the library is contaminated, or not as diverse as you expected.

FastQC lists all of the sequences which make up more than 0.1% of the total. To conserve memory only sequences which appear in the first 100,000 sequences are tracked to the end of the file. It is therefore possible that a sequence which is overrepresented but doesn't appear at the start of the file for some reason could be missed by this module.

Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).

Adapter Content

The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position.

Note that only samples with ≥ 0.1% adapter contamination are shown.

There may be several lines per sample, as one is shown for each adapter detected in the file.

From the FastQC Help:

The plot shows a cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. Once a sequence has been seen in a read it is counted as being present right through to the end of the read so the percentages you see will only increase as the read length goes on.

No samples found with any adapter contamination > 0.1%

Status Checks

Status for each FastQC section showing whether results seem entirely normal (green), slightly abnormal (orange) or very unusual (red).

FastQC assigns a status for each section of the report. These give a quick evaluation of whether the results of the analysis seem entirely normal (green), slightly abnormal (orange) or very unusual (red).

It is important to stress that although the analysis results appear to give a pass/fail result, these evaluations must be taken in the context of what you expect from your library. A 'normal' sample as far as FastQC is concerned is random and diverse. Some experiments may be expected to produce libraries which are biased in particular ways. You should treat the summary evaluations therefore as pointers to where you should concentrate your attention and understand why your library may not look random and diverse.

Specific guidance on how to interpret the output of each module can be found in the relevant report section, or in the FastQC help.

In this heatmap, we summarise all of these into a single heatmap for a quick overview. Note that not all FastQC sections have plots in MultiQC reports, but all status checks are shown in this heatmap.

Toggle navigation v1.9

SeA-SnaP mapping pipeline QC report

MultiQC Toolbox

Apply Highlight Samples

Apply Rename Samples

Apply Show / Hide Samples

Export Plots

Choose Plots

Save Settings

Load Settings

About MultiQC

SeA-SnaP mapping pipeline QC report run at the BIH CUBI

General Statistics

General Statistics: Columns

QualiMap

Coverage histogram Help

Cumulative genome coverage Help

Insert size histogram Help

GC content distribution Help

Genomic origin of reads Help

Gene Coverage Profile Help

RNA-SeQC

Transcript-associated reads Help

Strand Specificity Help

Read Counts Help

RSeQC

Infer experiment

featureCounts

Picard

Mark Duplicates Help

Samtools

Percent Mapped Help

Alignment metrics

Samtools Flagstat

XY counts

Mapped reads per contig

STAR

Alignment Scores

Gene Counts

FastQC

Sequence Counts Help

Sequence Quality Histograms Help

Per Sequence Quality Scores Help

Per Base Sequence Content Help

Rollover for sample name

Per Sequence GC Content Help

Per Base N Content Help

Sequence Length Distribution

Sequence Duplication Levels Help

Overrepresented sequences Help

Adapter Content Help

Status Checks Help

v1.9

Highlight Samples

Rename Samples

Show / Hide Samples

SeA-SnaP mapping pipeline QC report
run at the BIH CUBI

Coverage histogram

Cumulative genome coverage

Insert size histogram

GC content distribution

Genomic origin of reads

Gene Coverage Profile

Transcript-associated reads

Strand Specificity

Read Counts

Mark Duplicates

Percent Mapped

Sequence Counts

Sequence Quality Histograms

Per Sequence Quality Scores

Per Base Sequence Content

Per Sequence GC Content

Per Base N Content

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Status Checks